In situ PCR. Overview of procedures and applications.

  title={In situ PCR. Overview of procedures and applications.},
  author={Carlos A. Muro-Cacho},
  journal={Frontiers in bioscience : a journal and virtual library},
The evaluation of gene expression in the context of cellular morphology is essential to the full understanding of cell biology. A variety of methods for detection of nucleic acids are currently available. Solution PCR requires disruption of the sample and detection of the amplified material by electrophoresis in agarose gels. In situ Hybridization methods, on the other hand, permit morphological correlation and provide a high sensitivity that is sufficient for many applications. In some… CONTINUE READING