In situ-PCR for the detection of Mycobacterium paratuberculosis DNA in paraffin-embedded tissues.

@article{Sanna2000InSF,
  title={In situ-PCR for the detection of Mycobacterium paratuberculosis DNA in paraffin-embedded tissues.},
  author={Eleonora Sanna and Charlotte Woodall and N. J. Watt and Christopher J. Clarke and Marco Pittau and Antonio Leoni and Antonio M Nieddu},
  journal={European journal of histochemistry : EJH},
  year={2000},
  volume={44 2},
  pages={179-84}
}
Molecular biological techniques have permitted the rapid and sensitive detection of the Mycobacterium paratuberculosis genome in infected tissues, most commonly by polymerase chain reaction amplification of sequences in the IS900 DNA insertion sequence. The aim of this work was the detection of M. paratuberculosis DNA in ovine tissues by in situ-polymerase chain reaction, which is sensitive and localises the signal within the tissue sample. Paraffin embedded tissues from three acid-fast… CONTINUE READING

Similar Papers

Loading similar papers…