In silico rational design and systems engineering of disulfide bridges in the catalytic domain of an alkaline α-amylase from Alkalimonas amylolytica to improve thermostability.

@article{Liu2014InSR,
  title={In silico rational design and systems engineering of disulfide bridges in the catalytic domain of an alkaline α-amylase from Alkalimonas amylolytica to improve thermostability.},
  author={Long Liu and Zhuangmei Deng and Haiquan Yang and Jianghua Li and Hyun-dong Shin and Rachel R. Chen and Guocheng Du and Jian Chen},
  journal={Applied and environmental microbiology},
  year={2014},
  volume={80 3},
  pages={798-807}
}
High thermostability is required for alkaline α-amylases to maintain high catalytic activity under the harsh conditions used in textile production. In this study, we attempted to improve the thermostability of an alkaline α-amylase from Alkalimonas amylolytica through in silico rational design and systems engineering of disulfide bridges in the catalytic domain. Specifically, 7 residue pairs (P35-G426, Q107-G167, G116-Q120, A147-W160, G233-V265, A332-G370, and R436-M480) were chosen as… CONTINUE READING

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