In silico identification of lysocin biosynthetic gene cluster from Lysobacter sp. RH2180-5

@article{Panthee2017InSI,
  title={In silico identification of lysocin biosynthetic gene cluster from Lysobacter sp. RH2180-5},
  author={Suresh Panthee and Hiroshi Hamamoto and Yutaka Suzuki and Kazuhisa Sekimizu},
  journal={The Journal of Antibiotics},
  year={2017},
  volume={70},
  pages={204-207}
}
In silico identification of lysocin biosynthetic gene cluster from Lysobacter sp. RH2180-5 
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16 Citations
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Methods Citations
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16 Citations

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Citation Type

Identification of the biosynthetic gene cluster for the anti-MRSA lysocins through gene cluster activation using strong promoters of housekeeping genes and production of new analogs in Lysobacter sp. 3655.
TLDR
With readily detectable lysocins production in the engineered strains, it is shown that lysOCin production was abolished in the gene deletion mutant and then restored in the complementary strain, even when grown in conditions that did not support the wild type for lysoin production. Expand
Development of Novel Antibiotic Lysocin E Identified by Silkworm Infection Model.
TLDR
Lysocin E was identified by screening for therapeutic effectiveness in a silkworm Staphylococcus aureus infection model and has remarkable features compared with known antibiotics such as a novel mechanism of action and target. Expand
The Role of Amino Acid Substitution in HepT Toward Menaquinone Isoprenoid Chain Length Definition and Lysocin E Sensitivity in Staphylococcus aureus
TLDR
HepT was responsible for the definition of isoprenoid chain length of MKs and antibiotic sensitivity, and it was revealed that Smith strain possessed the classical pathway for MK biosynthesis like the other S. aureus strains. Expand
Complete Genome Sequence of Weissella hellenica 0916-4-2 and Its Comparative Genomic Analysis
TLDR
The availability of the complete genome of W. hellenica 0916-4-2 will facilitate further comparative genomic analysis of Weissella species, including studies of its biotechnological potential and improving the nutritional value of various food products. Expand
Large-scale screening and identification of novel pathogenic Staphylococcus aureus genes using a silkworm infection model.
TLDR
This study screened 380 genes whose function was unassigned utilizing gene-disrupted transposon mutants of the community-acquired methicillin-resistant S. aureus USA300 for pathogenicity using silkworms and identified 10 strains with reduced silkworm killing ability. Expand
Alteration of menaquinone isoprenoid chain length and antibiotic sensitivity by single amino acid substitution in HepT
TLDR
HepT, a polyprenyl diphosphate synthase involved in chain elongation of isoprenoid, in Smith strain was unique with a Q25P substitution, which led to the production of MK-10 and an increased sensitivity towards lysocin E. Expand
Antibiotics from Gram-negative bacteria: a comprehensive overview and selected biosynthetic highlights.
TLDR
A comprehensive list of natural products with antimicrobial activity from Gram-negative bacteria, including information on their biosynthetic origin(s) and molecular target(s), where known. Expand
Lysobacter species: a potential source of novel antibiotics
TLDR
The structural diversity, activity and biosynthesis of lysobacterial antibiotics are summarized and the importance of Lysobacter species for antibiotic production is highlighted. Expand
Uncovering Staphylococcus aureus genes with roles in pathogenicity by silkworm infection model
TLDR
Nine of the eleven genes identified were involved in virulence in mice and were previously not known to aggravate virulence of S. aureus and suggest that silkworm model is suitable for quantitative measurement of virulence, which is shared between silkworms and mammals. Expand
A Novel Spiro-Heterocyclic Compound Identified by the Silkworm Infection Model Inhibits Transcription in Staphylococcus aureus
TLDR
It is revealed that GPI0363 could bind to SigA, inhibit promoter-specific transcription in vitro, and prolong the survival of mice infected with methicillin-resistant S. aureus infections. Expand
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