In planta gene targeting.

@article{Fauser2012InPG,
  title={In planta gene targeting.},
  author={Friedrich Fauser and Nadine Roth and Michael Pacher and Gabriele Ilg and Roc{\'i}o S{\'a}nchez-Fern{\'a}ndez and Christian Biesgen and Holger Puchta},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2012},
  volume={109 19},
  pages={7535-40}
}
The development of designed site-specific endonucleases boosted the establishment of gene targeting (GT) techniques in a row of different species. However, the methods described in plants require a highly efficient transformation and regeneration procedure and, therefore, can be applied to very few species. Here, we describe a highly efficient GT system that is suitable for all transformable plants regardless of transformation efficiency. Efficient in planta GT was achieved in Arabidopsis… CONTINUE READING

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