In ovo live imaging of avian embryos.

  title={In ovo live imaging of avian embryos.},
  author={Paul M Kulesa and Caleb Michael Bailey and Cameron Cooper and Scott E. Fraser},
  journal={Cold Spring Harbor protocols},
  volume={2010 6},
Vertebrate development is best studied in an intact embryo model, but a robust interface between time-lapse microscopy and in vivo embryo health and maintenance can be difficult to achieve in model systems that rely on external factors for life support. This protocol presents a system for in ovo culture and time-lapse imaging of fluorescently labeled cells within living avian embryos, using a Teflon membrane that is oxygen-permeable and liquid-impermeable. The protocol describes the Teflon… CONTINUE READING

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