In-line SPE-CE using a fritless bead string design--application for the analysis of organic sulfonates including inline SPE-CE-MS for APTS-labeled glycans.

@article{Jooss2014InlineSU,
  title={In-line SPE-CE using a fritless bead string design--application for the analysis of organic sulfonates including inline SPE-CE-MS for APTS-labeled glycans.},
  author={Kevin Jooss and Johannes Sommer and Svenja-Catharina Bunz and Christian Neusuess},
  journal={Electrophoresis},
  year={2014},
  volume={35 9},
  pages={1236-43}
}
Despite many advantages like high separation efficiency CE comprises the main limitation of low concentration sensitivity, when compared to HPLC. In-line SPE is an efficient way to increase concentration sensitivity. Here, a fritless in-line-SPE-CE-MS method was developed in order to analyze anions of strong acids. Mixed-mode (weak anion exchange and RP) particles were used for enrichment and an acidic BGE was applied for separation. Different particle and capillary sizes were tested. A novel… CONTINUE READING