In-house HIV-1 RNA real-time RT-PCR assays: principle, available tests and usefulness in developing countries.

@article{Rouet2008InhouseHR,
  title={In-house HIV-1 RNA real-time RT-PCR assays: principle, available tests and usefulness in developing countries.},
  author={François Rouet and Herv{\'e} Ignace Eby Menan and Johannes Viljoen and Nicole Ngo-Giang-Huong and Kishorchandra N Mandaliya and Diane Val{\'e}a and Truong Thi Xuan Lien and Sivapragashini Danaviah and Dominique Rousset and Amandine Ganon and Eric Nerrienet},
  journal={Expert review of molecular diagnostics},
  year={2008},
  volume={8 5},
  pages={
          635-50
        }
}
The principle of currently available licensed HIV-1 RNA assays is based on real-time technologies that continuously monitor the fluorescence emitted by the amplification products. Besides these assays, in-house quantitative (q) real-time reverse transcription (RT)-PCR (RT-qPCR) tests have been developed and evaluated particularly in developing countries, for two main reasons. First, affordable and generalized access to HIV-1 RNA viral load is urgently needed in the context of expected universal… CONTINUE READING

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