In-depth mapping of the mouse brain N-glycoproteome reveals widespread N-glycosylation of diverse brain proteins

@inproceedings{Fang2016IndepthMO,
  title={In-depth mapping of the mouse brain N-glycoproteome reveals widespread N-glycosylation of diverse brain proteins},
  author={Pan Fang and Xin-jian Wang and Yu Xue and Ming-qi Liu and Wen-Feng Zeng and Yang Zhang and Lei Zhang and Xing Gao and Guo-quan Yan and Jun Yao and Hua-li Shen and Pengyuan Y. Yang},
  booktitle={Oncotarget},
  year={2016}
}
N-glycosylation is one of the most prominent and abundant posttranslational modifications of proteins. It is estimated that over 50% of mammalian proteins undergo glycosylation. However, the analysis of N-glycoproteins has been limited by the available analytical technology. In this study, we comprehensively mapped the N-glycosylation sites in the mouse brain proteome by combining complementary methods, which included seven protease treatments, four enrichment techniques and two fractionation… CONTINUE READING
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