In-depth analysis of protein phosphorylation by multidimensional ion exchange chromatography and mass spectrometry.

Abstract

Protein phosphorylation controls fundamental biological functions that are often deregulated in disease. Therefore, system-level understanding of complex pathophysiological processes requires methods that can be used to profile and quantify protein phosphorylation as comprehensively as possible. Here we present a detailed protocol to enrich phosphopeptides from total cell lysates in a form amenable to downstream analysis by mass spectrometry. Using these techniques, we have detected several thousands of phosphorylation sites in the NIH-3T3 cell line.

DOI: 10.1007/978-1-60761-780-8_6

Cite this paper

@article{Alcolea2010IndepthAO, title={In-depth analysis of protein phosphorylation by multidimensional ion exchange chromatography and mass spectrometry.}, author={Maria Pilar Alcolea and Pedro R Cutillas}, journal={Methods in molecular biology}, year={2010}, volume={658}, pages={111-26} }