In-cell solid-state NMR as a tool to study proteins in large complexes.

@article{Reckel2012IncellSN,
  title={In-cell solid-state NMR as a tool to study proteins in large complexes.},
  author={Sina Reckel and J. J Nieves L{\'o}pez and Frank Loehr and Clemens Glaubitz and Volker D{\"o}tsch},
  journal={Chembiochem : a European journal of chemical biology},
  year={2012},
  volume={13 4},
  pages={
          534-7
        }
}
A major limitation of solution NMR is molecular tumbling, which is often too slow for detection. Here we demonstrate that solid-state NMR spectroscopy in combination with flash freezing of cells can be used to detect proteins in the cellular environment and provides information on backbone chemical shifts. 
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