In Vivo Mechanisms Underlying Dopamine Release from Rat Nigrostriatal Terminals: II. Studies Using Potassium and Tyramine

  title={In Vivo Mechanisms Underlying Dopamine Release from Rat Nigrostriatal Terminals: II. Studies Using Potassium and Tyramine},
  author={Iain S. Fairbrother and Gordon William Arbuthnott and John S. Kelly and Steven P. Butcher},
  journal={Journal of Neurochemistry},
Abstract: The brain microdialysis technique has been used to examine the in vivo effects of potassium and tyramine on dopamine (DA) release and metabolism in the striatum of halothane‐anaesthetised rats. Increasing the concentration of potassium perfusing the dialysis probe (30–120 mM) induced a dose‐related efflux of DA. A dose‐related release of DA was also observed following addition of tyramine (1–100 μM) to the perfusing buffer. High concentrations of potassium were found to reduce the… 
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Use of rapid superfusion to differentiate the release of dopamine from striatal tissue induced by sympathomimetic amines from release induced by potassium.
It appears that all three of these agents induce blockade at the same site(s) and all of these data are in direct accord with the facilitated exchange diffusion mechanism proposed for the actions of sympathomimetic amines at DA nerve terminals.
The effect of tyramine, amphetamine and metaraminol on the metabolic disposition of 3 H-norepinephrine released from the adrenergic neuron.
  • F. LeitzF. Stefano
  • Biology, Chemistry
    The Journal of pharmacology and experimental therapeutics
  • 1971
The ability of each sympathomimetic amine to increase the outflow of 3H-NE from the perfused heart was found to correlate roughly with its ability to inhibit MAO activity in vitro in a homogenate of heart.
Initial release of [3H]dopamine from rat striatal synaptosomes: correlation with calcium entry
  • P. DrapeauM. Blaustein
  • Biology
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1983
It is concluded that Ca entry is a limiting step for DA release in synaptosomes during the first few seconds of depolarization, and the small size of the terminals in the mammalian central nervous system precludes intraterminal microelectrode recording.