In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes

@article{Nolan2016InVF,
  title={In Vivo Flow Cytometry of Circulating Tumor-Associated Exosomes},
  author={Jacqueline Nolan and M. Sarimollaoglu and D. Nedosekin and A. Jamshidi‐Parsian and E. Galanzha and R. Kore and R. Griffin and V. Zharov},
  journal={Analytical Cellular Pathology (Amsterdam)},
  year={2016},
  volume={2016}
}
Circulating tumor cells (CTCs) demonstrated the potential as prognostic markers of metastatic development. However, the incurable metastasis can already be developed at the time of initial diagnosis with the existing CTC assays. Alternatively, tumor-associated particles (CTPs) including exosomes can be a more valuable prognostic marker because they can be released from the primary tumor long before CTCs and in larger amount. However, little progress has been made in high sensitivity detection… Expand
Detection of Apoptotic Circulating Tumor Cells Using in vivo Fluorescence Flow Cytometry
TLDR
A novel approach toward in situ characterization of CTC apoptosis status using a multicolor in vivo flow cytometry platform with fluorescent detection for the real‐time identification and enumeration of such cells directly in blood flow is introduced. Expand
Advances of In Vivo Flow Cytometry on Cancer Studies
TLDR
In vivo flow cytometry (IVFC) is a burgeoning technique that allows noninvasive detection of CTCs in vivo and this powerful tool has been applied widely in many areas of cancer‐related studies, especially the CTC studies. Expand
December 2017 Fluorescence detection , enumeration and characterization of single circulating cells in vivo : technology , applications and future prospects
There are many diseases and biological processes that involve circulating cells in the bloodstream, such as cancer metastasis, immunology, reproductive medicine, and stem cell therapies. This hasExpand
Fluorescence detection, enumeration and characterization of single circulating cells in vivo: technology, applications and future prospects.
TLDR
Recent technical advances in IVFC are reviewed, with emphasis on instrumentation, contrast mechanisms, and detection sensitivity, and key applications in biomedical research are described, including cancer research and immunology. Expand
In vivo liquid biopsy using Cytophone platform for photoacoustic detection of circulating tumor cells in patients with melanoma
TLDR
The high sensitivity of the Cytophone technology using an in vivo photoacoustic flow cytometry platform with a high pulse rate laser and focused ultrasound transducers for label-free detection of melanin-bearing CTCs in patients with melanoma is demonstrated. Expand
Fluorescence detection, enumeration and characterization of single circulating cells in vivo: technology, applications and future prospects.
TLDR
Recent technical advances in IVFC are reviewed, with emphasis on instrumentation, contrast mechanisms, and detection sensitivity, and key applications in biomedical research are described, including cancer research and immunology. Expand
Real‐time quantitative PCR detection of circulating tumor cells using tag DNA mediated signal amplification strategy
TLDR
A real‐time quantitative PCR (Q‐PCR) based method for sensitive detection of CTCs without DNA extraction is established and can be a good alternative for the determination of cancer progress. Expand
Dynamic blood flow phantom for in vivo liquid biopsy standardization
TLDR
A dynamic cell flow phantoms with appropriate biochemical, optical, thermal, and acoustic properties can be promising for the establishment of standardization tool for calibration of PA, fluorescent, Raman, and other detection methods of in vivo flow cytometry and liquid biopsy. Expand
New Frontiers in Diagnosis and Therapy of Circulating Tumor Markers in Cerebrospinal Fluid In Vitro and In Vivo
TLDR
This review outlines the advantages, limitations and clinical utility of emerging liquid biopsy in vitro and photoacoustic flow cytometry (PAFC) in vivo for assessment of CSF markers including circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), microRNA, proteins, exosomes and emboli. Expand
Optimized signal detection and analysis methods for in vivo photoacoustic flow cytometry
Melanoma is known as a malignant tumor of melanocytes, which usually appear in the blood circulation at the metastasis stage of cancer. Thus the detection of circulating melanoma cells is useful forExpand
...
1
2
...

References

SHOWING 1-10 OF 45 REFERENCES
Detecting Circulating Tumor Cells: Current Challenges and New Trends
TLDR
A thorough review of the current knowledge and technology of CTC detection will assist the scientific community in the development of more efficient CTC assay systems. Expand
Isolation of circulating tumor cells using a microvortex-generating herringbone-chip
TLDR
A high-throughput microfluidic mixing device, the herringbone-chip, or “HB-Chip,” is described, which provides an enhanced platform for CTC isolation and reveals microclusters of CTCs, previously unappreciated tumor cell aggregates that may contribute to the hematogenous dissemination of cancer. Expand
In vivo, noninvasive, label-free detection and eradication of circulating metastatic melanoma cells using two-color photoacoustic flow cytometry with a diode laser.
TLDR
Advances in in vivo photoacoustic blood cancer testing with a high-pulse-repetition-rate diode laser make feasible the early diagnosis of melanoma during the initial parallel progression of primary tumor and CTCs, and laser blood purging using noninvasive or hemodialysis-like schematics for the prevention of metastasis. Expand
Photoacoustic and photothermal detection of circulating tumor cells, bacteria and nanoparticles in cerebrospinal fluid in vivo and ex vivo.
TLDR
Novel CSF tests based on in vivo photoacoustic flow cytometry (PAFC) and ex vivo photothermal scanning cytometry are introduced and its translation for use in humans is expected to improve disease diagnosis beyond conventional detection limits. Expand
Nanoparticle analysis of circulating cell-derived vesicles in ovarian cancer patients.
TLDR
It is demonstrated that NTA can measure the size distributions of cell-derived vesicles comparable to other analysis instrumentation, and the use of fluorescent-labeled antibodies against specific markers with NTA allows the determination of the "phenotype" of the cell- derived vesicle concentration. Expand
New technologies for the detection of circulating tumour cells.
TLDR
This review will focus on the techniques currently used, the technical advancements made, the limitations of CTC detection and future perspectives in this field. Expand
Circulating Tumor Cells
TLDR
The purpose of this book chapter is to describe the use of the 4-channel CMx (cells captured in maximum) microfluidic chip, containing special micropatterns coated with an antibodyconjugated supported lipid bilayer (SLB) on its surface, to capture and isolate CTCs from the blood of cancer patients. Expand
In vivo flow cytometry: A horizon of opportunities
Flow cytometry (FCM) has been a fundamental tool of biological discovery for many years. Invasive extraction of cells from a living organism, however, may lead to changes in cell properties andExpand
In vivo magnetic enrichment and multiplex photoacoustic detection of circulating tumour cells.
TLDR
This approach allows circulating tumour cells to be concentrated from a large volume of blood in the vessels of tumour-bearing mice, and this could have potential for the early diagnosis of cancer and the prevention of metastasis in humans. Expand
Circulating tumor cells--new challenges ahead.
TLDR
The evaluation of C TCs now represents a promising new diagnostic tool, especially for advanced-stage cancer patients, for whom CTCs can be used as a “liquid biopsy” that allows physicians to follow cancer changes over time and tailor treatment accordingly. Expand
...
1
2
3
4
5
...