In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones.

  title={In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones.},
  author={Louis Masclef and Uriarte Maxime and Oumaima Ahmed and Nadine Sen Nkwe and Haithem Barbour and Nicholas Victor Iannantuono and Amina Mama Boubekeur and Salima Daou and El Bachir Affar},
  journal={Journal of visualized experiments : JoVE},
Ubiquitination is a post-translational modification that plays important roles in various signaling pathways and is notably involved in the coordination of chromatin function and DNA-associated processes. This modification involves a sequential action of several enzymes including E1 ubiquitin-activating, E2 ubiquitin-conjugating and E3 ubiquitin-ligase and is reversed by deubiquitinases (DUBs). Ubiquitination induces degradation of proteins or alteration of protein function including modulation… 

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