In Vitro Synthesis of Enzymes of the Tryptophan Operon of Escherichia coli ( anthranilate synthetase / tryptophan synthetase / DNA-dependent protein synthesis )

  • P. H. POUWELS AND J. VAN ROTTERDAM
Two active enzymes of the tryptophan operon of E. coli, anthranilate synthetase (product of trp E and trp D) and tryptophan synthetase (EC 4.2.1.20) (product of trp B and trp A), have been synthesized in a DNA-dependent preparation for protein synthesis. Anthranilate synthetase and the two components of tryptophan synthetase (tryptophan synthetase A and B… CONTINUE READING