In Vitro Spoilation of Silicone-Hydrogel Soft Contact Lenses in a Model-Blink Cell

  title={In Vitro Spoilation of Silicone-Hydrogel Soft Contact Lenses in a Model-Blink Cell},
  author={Cheng-Chun Peng and Neil P. Fajardo and Trust T. Razunguzwa and Clayton J. Radke},
  journal={Optometry and Vision Science},
PurposeWe developed an in vitro model-blink cell that reproduces the mechanism of in vivo fouling of soft contact lenses. In the model-blink cell, model tear lipid directly contacts the lens surface after forced aqueous rupture, mirroring the pre–lens tear-film breakup during interblink. MethodsSoft contact lenses are attached to a Teflon holder and immersed in artificial tear solution with protein, salts, and mucins. Artificial tear-lipid solution is spread over the air/tear interface as a… 
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Differential Deposition of Fluorescently Tagged Cholesterol on Commercial Contact Lenses Using a Novel In Vitro Eye Model
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An imaging-based analysis of lipid deposits on contact lens surfaces.
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    Contact lens & anterior eye : the journal of the British Contact Lens Association
  • 2017
Fouling in ocular devices: implications for drug delivery, bioactive surface immobilization, and biomaterial design.
From the earliest implanted horsehair and silk seton for glaucoma drainage to the recent mini telescopes for sight recovery, this review provides a concise incursion into the gradual evolution of biomaterials for the design of implantable ocular devices as well as approaches used to overcome the challenges with fouling.


Protein deposition on contact lenses: the past, the present, and the future.
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  • Physics, Biology
    Contact lens & anterior eye : the journal of the British Contact Lens Association
  • 2012
Lipid Adsorption onto Hydrogel Contact Lens Materials. Advantages of Nile Red over Oil Red O in Visualization of Lipids
It was found that lipids adsorb to hydrogel materials quite readily either in a pure state or combined with mucin or other proteins.
The adsorption of major tear film lipids in vitro to various silicone hydrogels over time.
In vitro lipid adsorption varied greatly depending on the lens material for both polar and nonpolar lipids, and there was less in vitro Adsorption of lipid to the lotrafilcon A and B polymers than for any of the other silicone hydrogel polymers tested.
In vitro lipid deposition on hydrogel and silicone hydrogel contact lenses.
Hydrogel and most silicone hydrogels appear to adsorb lipids relatively quickly (i.e., within the first day).
Lysozyme and Lipid Deposition on Silicone Hydrogel Contact Lens Materials
The quantity and conformation of lysozyme and the quantity of lipid deposited on hydrogel contact lenses is significantly influenced by the composition of the lens material.
A Method of Imaging Lipids on Silicone Hydrogel Contact Lenses
Nile Red and Oil Red O are both able to detect lipids on soft lenses in both in vitro and ex vivo conditions, and oil Red O appears to be a better stain for silicone hydrogel lenses as it offers a higher signal to noise ratio.
Visualizing hydrophobic domains in silicone hydrogel lenses with Sudan IV.
Differences in staining response after exposure to wear and cleaning conditions indicate the potential for protein and lipid deposition on the different lens types and the ability of MPS to affect that deposition.
Lipid deposition on hydrogel contact lenses: how history can help us today.
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    Optometry and vision science : official publication of the American Academy of Optometry
  • 2007
The composition of the tear film lipids and their interaction with contact lens materials are described, with a particular emphasis on how the chemistry of novel silicone hydrogel materials has resulted in clinicians needing to understand the deposition of lipids onto contact lenses and how they may best manage this complication.
Protein-lipid interaction on the surface of a rigid gas-permeable contact lens in vitro.
The hydrophobic nature of rigid gas-permeable contact lenses causes them to be more prone to lipid than to protein deposition, and the bound deposited hydrophilic protein then alters subsequent binding of both protein and lipid.