In Vitro Propagation of an Edible Bamboo Bam- busa Bambos and Assessment of Clonal Fidelity through Molecular Markers

@article{Anand2013InVP,
  title={In Vitro Propagation of an Edible Bamboo Bam- busa Bambos and Assessment of Clonal Fidelity through Molecular Markers},
  author={M. Anand and Jasmine Brar and Anil K. Sood},
  journal={Journal of medical and bioengineering},
  year={2013},
  volume={2},
  pages={257-261}
}
 Abstract—An efficient and reproducible protocol has been established through the technique of forced axillary branching for the propagation of an important edible bamboo species namely Bambusa bambos. High frequency multiple shoot induction was achieved from nodal segments collected from elite genotype on Murashige and Skoog’s (MS) medium supplemented with 4.4 µM Benzylaminopurine (BAP) and 1.16 µM Kinetin (Kn). The size of explant and season greatly influenced the frequency of bud break… 

Figures and Tables from this paper

Development of efficient micropropagation protocol through axillary shoot proliferation for Bambusa vulgaris ‘wamin’ and Bambusa bambos and assessment of clonal fidelity of the micropropagated plants through Random Amplified Polymorphic DNA markers

The clonal fidelity of in vitro regenerated plantlets was assessed using 10 random amplified polymorphic DNA (RAPD) markers and all the bands generated using the 10 RAPD decamers were monomorphic and produced more than six scorable bands within the band size of 240–2,505 base pairs.

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

The highest 100% survivability was recorded from well-established rooted plantlets and the regenerated plants showed well developed root and shoot systems in field condition.

In vitro Propagation of an Edible Bamboo Dendrocalamus latiflorus Munro Using Nodal Explants

An efficient and reproducible protocol for mass propagation of an important edible bamboo species Dendrocalamus latiflorus Munro has been developed through in vitro culture. Nodal segments containing

In vitro propagation of bamboo species through axillary shoot proliferation: a review

All identified cases, in which development of axillary shoots was used to propagate bamboo plants are listed, by presenting successful ways for disinfection, in vitro bud sprouting, multiplication, rooting and acclimatization.

Assessment of Genetic Stability of Micropropagated Bambusa balcooa Roxb. using RAPD Marker

Bambusa balcooa Roxb. was in vitro propagated by optimizing protocol using nodal segment from secondary branches with 100% success in MS liquid media containing 100 mg/l Myo-inositol, 3% sucrose

Clonal bamboo production based on in vitro culture

Both rooting inductionculture medium and mini-incubator system use were effective in enabling adventitious root formation and the presence of activated carbon in the rooting induction culture medium resulted in a higher clonal plant survival rate.

Analysis of diversity using simple sequence repeat (SSR): distinctions between original Parmentiera cereifera tree and somaclones

Micropropagation is a safe mode for multiplication of true-to-type plants of P. cereifera in vitro and the results revealed the increase in the degree of similarity between the tested plants in the SSR analyses.

Elimination and molecular identification of endophytic bacterial contaminants during in vitro propagation of Bambusa balcooa

To the authors' knowledge, this is the first report for B. balcooa where antibiotics were used as surface sterilant as well as medium component, to control endophytic bacterial contaminants, followed by their identification.

Regeneration Technique of Bamboo Species through Nodal Segments: A Review

This review intends to explore an overview of the recent literature reports to summarize the importance of micropropagation by using nodal segments of bamboo species and factors influencing it.

Enhanced rhizome induction and fast regeneration protocol in liquid culture of Dendrocalamus longispathus Kurz: A single step culture

The present experimental study was aimed for in-vitro regeneration through nodal culture of Dendrocalamus longispathus an important bamboo species of north-eastern region. Single auxillary buds were

References

SHOWING 1-10 OF 17 REFERENCES

In vitro micropropagation of Bambusa balcooa Roxb. through nodal explants from field-grown culms and scope for upscaling.

An efficient and reproducible in vitro procedure for large-scale multiplication of Bambusa balcooa Roxb has been described and the tech-economic viability was studied with consideration that the production will be executed in a well-established plant tissue culture laboratory.

Clonal Propagation and Production of Genetically Uniform Regenerants from Axillary Meristems of Adult Bamboo

  • M. DasA. Pal
  • Biology
    Journal of Plant Biochemistry and Biotechnology
  • 2012
The axillary bud-break and multiple bud induction were obtained from the nodal explants of field-grown culms of Bambusa tulda in liquid Murashige and Skoog’s (MS) basal medium and the clonal fidelity of the regenerants was established by RAPD analysis advocating clonal propagation through axillary meristem culture.

In vitro plant regeneration via callogenesis and organogenesis in Bambusa vulgaris

Friable calli were induced from mature excised shoots of Bambusa vulgaris on Murashige and Skoog's (MS) medium supplemented with 2.2 µM6-benzylamino-purine (BAP), 9.04 µM 2,4-dichlorophenoxyacetic acid and 3.76 µM indole-3-butyric acid with the regeneration of shoot buds from calli confirmed.

Evaluation of clonal fidelity of in vitro raised plants of Guadua angustifolia Kunth using DNA-based markers

All banding profiles from micropropagated plants were monomorphic and similar to those of the mother plant, thus ascertaining the true nature of the in vitro raised plants.

In vitro seed germination of economically important edible bamboo Dendrocalamus membranaceus Munro.

Conclusively, in vitro propagation protocol developed in D. membranaceus using mature seeds as an explants is reported for the first time and will ensure regeneration of large number of plants in a relatively short time.

Ascertaining clonal fidelity of tissue culture raised plants of Bambusa balcooa Roxb. using inter simple sequence repeat markers

Inter simple sequence repeat (ISSR) marker assay was employed to validate the clonal fidelity of in vitro raised Bambusa balcooa plantlets multiplied by enhanced axillary proliferation up to 33 passages confirming the genetic stability in the latter.

Somatic embryogenesis in bamboo

Bamboo is one of the most universally useful plant commodities known to man providing food, raw material, shelter, and even medicine for the greater part of the world’s population. However,

Shoot regeneration, re-flowering and post flowering survival in bamboo inflorescence culture

In vitro grown inflorescences of Bambusa edulis revealed that auxins and ACC could be significant growth regulators in this process and promoted shoot regeneration less effectively than NAA and 2,4-D.