In Vitro Parasiticidal Effect of Nitazoxanide against Echinococcus multilocularis Metacestodes

  title={In Vitro Parasiticidal Effect of Nitazoxanide against Echinococcus multilocularis Metacestodes},
  author={Marianne Stettler and Renate Fink and Mirjam Walker and Bruno Gottstein and Timothy G Geary and Jean François Rossignol and Andrew Hemphill},
  journal={Antimicrobial Agents and Chemotherapy},
  pages={467 - 474}
ABSTRACT When humans serve as inadvertent intermediate hosts for Echinococcus multilocularis, disease (alveolar echinococcosis [AE]) may result from the expanding parasite metacestode in visceral organs, mostly in the liver. Benzimidazole carbamate derivatives such as mebendazole and albendazole are used for chemotherapeutic treatment of AE. However, these treatments are, in most cases, parasitistatic rather than parasiticidal. As treatment is discontinued, a recurrence of parasite growth has… 

In vitro effects of nitazoxanide on Echinococcus granulosus protoscoleces and metacestodes.

In vitro efficacy of nitazoxanide, a broad-spectrum drug used against intestinal parasites and bacteria, was investigated and indicates a potential for nitaz oxanide as an alternative treatment option against CE.

ctivities of fenbendazole in comparison with albendazole against chinococcus multilocularis metacestodes in vitro and in a murine nfection model

Results indicate the potential usefulness of fenbendazole for the chemotherapy of AE and show drug-induced alterations within the germinal layer of the parasites and an almost complete absence of microtriches.

Alveolar and cystic echinococcosis: towards novel chemotherapeutical treatment options

New drugs are needed to cure AE and CE, of which the metacestode (larval) stages cause the neglected diseases cystic echinococcosis and alveolar echinOCoccosis, respectively.

Innovative chemotherapeutical treatment options for alveolar and cystic echinococcosis

The efforts toward obtaining better anti-parasitic compounds which have been undertaken to improve chemotherapeutical treatment of echinococcosis are reviewed, and the achievements in the field of host-Parasite interactions which may also lead to new immuno-therapeutical options are summarized.

In Vitro and In Vivo Efficacies of Mefloquine-Based Treatment against Alveolar Echinococcosis

In conclusion, mefloquine represents an interesting drug candidate for the treatment of AE, and these results should be followed up in appropriate in vivo studies.

Echinococcus metacestodes as laboratory models for the screening of drugs against cestodes and trematodes

This review will focus on E. granulosus and E. multilocularis metacestode laboratory models and will review the use of these models in the search for novel drugs that could be employed for chemotherapeutic treatment of echinococcosis.

In Vitro and In Vivo Activities of Dicationic Diguanidino Compounds against Echinococcus multilocularis Metacestodes

Thiophene-diguanidino derivatives with improved bioavailability when administered orally could lead to treatment options against alveolar echinococcosis.

Limitations of amphotericin B and nitazoxanide in the treatment of alveolar echinococcosis

A combination of AMB-D and nitazoxanide has been used to treat a case of AE for the first time, and the German patient who was treated with the combination had intrahepatic cholestasis and had previously developed recurrent toxic hepatitis under benzimidazole treatment.



Echinococcus multilocularis Alkaline Phosphatase as a Marker for Metacestode Damage Induced by In Vitro Drug Treatment with Albendazole Sulfoxide and Albendazole Sulfone

It is shown that the increase of E. multilocularis alkaline phosphatase activity in culture supernatants during in vitro drug treatment with albendazole derivatives correlates with the progressive degeneration and destruction of the metacestode tissue.

Efficacies of Albendazole Sulfoxide and Albendazole Sulfone against In Vitro-Cultivated Echinococcus multilocularis Metacestodes

The in vitro cultivation model presented here provides an ideal first-round test system for screening of antiparasite drugs and Transmission electron microscopic investigation of parasite tissues and nuclear magnetic resonance spectroscopy of vesicle fluids demonstrated that albendazole sulfoxide and albendsole sulfone had similar effects with respect to parasite ultrastructure and changes in metabolites in vesicles fluids.

Veterinary aspects of alveolar echinococcosis--a zoonosis of public health significance.

Immunology and morphology studies on the proliferation of in vitro cultivatedEchinococcus multilocularis metacestodes

The maintenance and proliferation of E. multilocularis metacestodes as well as the formation of protoscolices in a chemically defined medium devoid of host influence are described.

Nitazoxanide for the treatment of intestinal protozoan and helminthic infections in Mexico.

The lethal effect of mebendazole on secondary Echinococcus granulosus, cysticerci of Taenia pisiformis and tetrathyridia of Mesocestoides corti

The effectiveness of mebendazole seemed to depend on the formulation of the drug and its route of administration, and it has been shown that only a small amount of living germinative tissue is required to produce a new E. granulosus cyst.

Echinococcosis: an emerging or re-emerging zoonosis?

Proliferation and metastases formation of larval Echinococcus multilocularis. II. Ultrastructural investigations.

The larval stage (metacestode) of Echinococcus multilocularis was studied by means of electron microscopy before and after subcutaneous transplantation to jirds and in their lymph nodes and lungs with parasite metastases to unequivocally proven the cestode nature of the cellular protrusions.