In Vitro Manipulation of Cucumber ( Cucumis Sativus L . ) Pollen and Microspores : Isolation Procedures , Viability Tests , Germination , Maturation

In vitro germination of isolated pollen grains and maturation of microspores are needed for several biotechnological manipulations of cucumber pollen. The present study was focused on establishing optimal conditions for monitoring isolation, sterilization and viability. Germination of mature cucumber pollen and maturation of microspores were attempted. Five… (More)