In Vitro Glucuronidation of Wushanicaritin by Liver Microsomes, Intestine Microsomes and Expressed Human UDP-Glucuronosyltransferase Enzymes

@article{Hong2017InVG,
  title={In Vitro Glucuronidation of Wushanicaritin by Liver Microsomes, Intestine Microsomes and Expressed Human UDP-Glucuronosyltransferase Enzymes},
  author={X. Hong and Yuanru Zheng and Zifei Qin and Baojian Wu and Yi Dai and Hao Gao and Zhihong Yao and F. Gonzalez and X. Yao},
  journal={International Journal of Molecular Sciences},
  year={2017},
  volume={18}
}
Wushanicaritin, a natural polyphenol compound, exerts many biological activities. This study aimed to characterize wushanicaritin glucuronidation by pooled human liver microsomes (HLM), human intestine microsomes and individual uridine diphosphate-glucuronosyltransferase (UGT) enzyme. Glucuronidation rates were determined by incubating wushanicaritin with uridine diphosphoglucuronic acid-supplemented microsomes. Kinetic parameters were derived by appropriate model fitting. Reaction phenotyping… Expand
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Chemical inhibition and gene silencing results suggested that BCRP, MRP1, MRp3 and MRP4 were significant contributors to excretion of wushanicaritin glucuronides. Expand
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