In-Vitro Differentiation of Mature Dendritic Cells From Human Blood Monocytes

  title={In-Vitro Differentiation of Mature Dendritic Cells From Human Blood Monocytes},
  author={Robert K Gieseler and Dirk J Heise and Afsaneh Soruri and P. Schwartz and John A. Peters},
  journal={Developmental Immunology},
  pages={25 - 39}
Representing the most potent antigen-presenting cells, dendritic cells (DC) can now be generated from human blood monocytes. We recently presented a novel protocol employing GM-CSF, IL-4, and IFN-gamma to differentiate monocyte-derived DC in vitro. Here, such cells are characterized in detail. Cells in culture exhibited both dendritic and veiled morphologies, the former being adherent and the latter suspended. Phenotypically, they were CD1a-/dim, CD11a+, CD11b++, CD11c+, CD14dim/-, CD16a-/dim… CONTINUE READING


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