In Vitro DNase I Footprinting.

  title={In Vitro DNase I Footprinting.},
  author={Beno{\^i}t P Leblanc and Tom Moss},
  journal={Methods in molecular biology},
The association of proteins with the DNA double helix can interfere with the accessibility of the latter to nucleases. This is particularly true when using bulky nucleases such as DNase I. The DNase I footprinting method was developed to take advantage of this fact in the study of DNA-protein interactions: it consists in comparing the pattern of fragments… CONTINUE READING