In Vitro Cultivation of Microphallus turgidus (Trematoda: Microphallidae) from Metacercaria to Ovigerous Adult with Continuation of the Life Cycle in the Laboratory

  title={In Vitro Cultivation of Microphallus turgidus (Trematoda: Microphallidae) from Metacercaria to Ovigerous Adult with Continuation of the Life Cycle in the Laboratory},
  author={Oscar J. Pung and Ashley R. Burger and Michael F. Walker and Whitney L. Barfield and Micah H. Lancaster and Christina E. Jarrous},
  booktitle={The Journal of parasitology},
Abstract In vitro cultivation of trematodes would aid studies on the basic biology of the parasites and the development of chemotherapies and vaccines. Our goal was to measure the in vitro survival and maturation of metacercariae of Microphallus turgidus under different culture conditions. Metacercariae of M. turgidus from grass shrimp (Palaemonetes pugio) were excysted and cultured in humidified air at 37 C in RPMI-1640 medium supplemented with 20% calf, chicken, or horse serum. Deposition of… 
Optimization of Culture Conditions for In Vitro Fertilization and Reproduction of Microphallus turgidus (Trematoda: Microphallidae)
The reproducibility of the M. turgidus culture protocol was verified, optimal culture conditions were defined, and why the parasite can be grown successfully in the absence of the definitive host was investigated.
In vitro cultivation of Cymatocarpus solearis (Brachycoeliidae) metacercariae to obtain the adult stage without the marine turtle definitive host.
This study is significant because it is the first time that a digenean of the family Brachycoeliidae has been demonstrated to develop in vitro from metacercariae into adults capable of producing eggs using the yolk of unfertilized chicken eggs.
In Vitro Insemination of the Microphallid Digenean Gynaecotyla adunca
The length of time needed for in vitro sperm development in excysted metacercariae and whether the adult worms could self-inseminate in the absence of conspecifics are determined and provide the basis for a straightforward, reproducible procedure that permits the in vitro insemination of the parasite G. adunca.
Behavioral analysis of Microphallus turgidus cercariae in relation to microhabitat of two host grass shrimp species (Palaemonetes spp.).
Movement of the cercariae away from light into dark, active swimming at or near the bottom of the water column, and a lack of response to host odors suggest that the cerbariae utilize search patterns that place the parasite in the preferred microhabitat of the principle second intermediate host, the grass shrimp P. pugio.
Influence of a Trematode Parasite (Microphallus turgidus) on Grass Shrimp (Palaemonetes pugio) Response to Refuge and Predator Presence
Findings suggest that M. turgidus does not influence grass shrimp usage of CWD, but it alters shrimp swimming and backthrust behavior, thereby rendering infected shrimp more susceptible to predation.
Optimization of Conditions for In Vitro Culture of the Microphallid Digenean Gynaecotyla adunca
Worm longevity and egg production increased when worms were grown in DME/F-12 supplemented with 20% chicken, horse, or newborn calf serum but the greatest number of eggs was deposited in cultures containing horse or chicken serum.


In vitro cultivation of Maritrema novaezealandensis (Microphallidae): the effect of culture medium on excystation, survival and egg production
Egg production was highest in specimens cultured in media with a supplement of chicken serum and reached a maximum after 2 days of cultivation, however, growth did not occur after the first day of cultivation in any of the three media.
Strigeid trematodes (Cotylurus lutzi) cultured in vitro: production of normal eggs with continuance of life cycle.
Tetracotyles of Cotylurus lutzi were incubated to adults in a basic medium of 40% NCTC135 and 40% chicken serum, supplemented with 20% extract of chicken heart, liver, muscle, intestine wall, or
In vitro culture of the avian echinostome Himasthla elongata: from redia to marita.
Cultivation of helminths in chick embryos.
Flukes without snails: advances in the in vitro cultivation of intramolluscan stages of trematodes.
Recent advances in the cultivation of several larval trematode species are reviewed and the potential use of this culture system for addressing fundamental questions of host-parasite compatibility is discussed.
In vitro development of Haplorchis taichui (Trematoda: Heterophyidae).
RPMI 1640 with blood agar can be used as short-term maintenance for the in vitro culture of H. taichui, however, further studies are needed.
Results showed that E. cinetorchis cincariae could be cultured to viable metacercariae in an in vitro culture system and that 0.5× RPMI 1640 plus 10% fetal bovine serum was the most useful medium for cultivation.
In vitro excystation of metacercariae of Microphallus turgidus under different physicochemical conditions
Results indicate that such a combination of extrinsic factors is provided by the definitive host for successful excystation of M. turgidus.