In Vitro Cultivation of Microphallus turgidus (Trematoda: Microphallidae) from Metacercaria to Ovigerous Adult with Continuation of the Life Cycle in the Laboratory

@inproceedings{Pung2009InVC,
  title={In Vitro Cultivation of Microphallus turgidus (Trematoda: Microphallidae) from Metacercaria to Ovigerous Adult with Continuation of the Life Cycle in the Laboratory},
  author={O. Pung and Ashley R. Burger and M. F. Walker and Whitney L. Barfield and Micah H. Lancaster and Christina E. Jarrous},
  booktitle={The Journal of parasitology},
  year={2009}
}
Abstract In vitro cultivation of trematodes would aid studies on the basic biology of the parasites and the development of chemotherapies and vaccines. Our goal was to measure the in vitro survival and maturation of metacercariae of Microphallus turgidus under different culture conditions. Metacercariae of M. turgidus from grass shrimp (Palaemonetes pugio) were excysted and cultured in humidified air at 37 C in RPMI-1640 medium supplemented with 20% calf, chicken, or horse serum. Deposition of… Expand
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In vitro culture of marine trematodes from their snail first intermediate host.
Behavioral analysis of Microphallus turgidus cercariae in relation to microhabitat of two host grass shrimp species (Palaemonetes spp.).
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