In Vitro CuIture of the Tropical Sponge Axinella corrugata (Demospongiae): Effect of Food Cell Concentration on Growth, Clearance Rate, and Biosynthesis of Stevensine

@article{Duckworth2002InVC,
  title={In Vitro CuIture of the Tropical Sponge Axinella corrugata (Demospongiae): Effect of Food Cell Concentration on Growth, Clearance Rate, and Biosynthesis of Stevensine},
  author={Alan R. Duckworth and Gail A. Samples and Amy E Wright and Shirley A. Pomponi},
  journal={Marine Biotechnology},
  year={2002},
  volume={5},
  pages={519-527}
}
In vitro culture is one possible method for supplying sponge metabolites for pharmaceutical applications, but appropriate feeding regimens that maximize both growth and metabolite biosynthesis are largely unknown. According to the natural concentration (NC) of cells 1 to 50 µm in size that are available to wild Axinella corrugata, we fed explants a multispecific diet of bacteria, microalgae, and yeast at 4 different concentrations: 1NC, 3NC, 5NC, and 5+1NC (the last consisted of 5 NC of… CONTINUE READING
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