In Vitro Bactericidal Effectiveness of Four Aminoglycoside Antibiotics

  title={In Vitro Bactericidal Effectiveness of Four Aminoglycoside Antibiotics},
  author={P J Hyams and Michael S Simberkoff and James J. Rahal},
  journal={Antimicrobial Agents and Chemotherapy},
  pages={87 - 94}
The antibacterial activity of four aminoglycoside antibiotics (gentamicin, Sch 13706, tobramycin, and sisomicin) was tested against eight gram-negative and three gram-positive species. A total of 323 strains were studied by the broth dilution technique. Tobramycin and sisomicin had greater bacteriostatic and bactericidal activity against Pseudomonas strains than did gentamicin and Sch 13706. Of the four antibiotics, sisomicin was most active against Klebsiella, Enterobacter, Escherichia coli… 
Sisomicin: A composite of comparative in vitro studies spanning six years
Results from this and other in vitro studies conducted over the past six years suggest that sisomicin possesses certain advantages over other aminoglycosides that might be extrapolated to the in vivo situation.
In vitro studies with sisomicin, gentamicin and tobramycin
Sisomicin was the most active drug against Enterobacter aerogenes, Klebsiella pneumoniae, Serratia marcescens and Proteus mirabilis, while tobramycin was theMost active against Pseudomonas aeruginosa, in terms of inhibition and bactericidal activity.
A study of the potency of certain aminoglycoside antibiotics against some local clinical isolates.
  • M. Aziz, A. Ammar
  • Medicine
    JPMA. The Journal of the Pakistan Medical Association
  • 1983
The inhibitory activity of Kanamycin, Gentamicin, Tobramycin and Sisomicin was studied against some of the common local clinical isolates by disc diffusion technique, finding differences observed could be attributed to the possibility of existence of inherently resistant strains and geograaphical differences in the susceptibilities of the strains.
Sisomicin Treatment of Urinary Tract Infections
This new antibiotic has a spectrum of in vitro antimicrobial activity similar to that of gentamicin, however, it is more active particularly against strains of Pseudomonas aeruginosa and indole positive proteus.
Sisomicin: Antibacterial activity, pharmacokinetics, and a clinical trial
Sisomicin appears to be a favorable alternative to gentamicin, and the pharmacokinetics were found to be similar to those of other aminoglycosides and indicate satisfactory distribution in tissues and infection sites.
In Vitro Activity of Gentamicin, Amikacin, and Netilmicin Alone and in Combination with Carbenicillin Against Serratia marcescens
Clinically attainable subinhibitory concentrations of carbenicillin enhanced the activity of the three aminoglycosides against all isolates with MBCs of carpenicillin ≤2,000 μg/ml.
Aqueous kinetics of sisomicin sulphate
The agar diffusion technique of microbial assay was used to determine the aqueous penetration and bioavailability of a subconjunctivally placed standard dose of sisomicin sulphate in 20 human volunteers undergoing elective cataract surgery and the antibiotic was bio-available up to 1203 minutes after injection and easily covers the minimum inhibitory concentration of Staphylococcus aureus and Pseudomonas.
Sisomicin: a review of eight years' experience.
The pharmacokinetics and toxicity of sisomicin in humans appear to be similar to those of gentamicin, despite earlier reports of greater acute toxicity in animals, and has been shown to be effective for treatment of severe infections in humans, including some infections caused by gentamicIn-resistant bacteria.
Antibacterial Activity of Netilmicin, a New Aminoglycoside Antibiotic, Compared with That of Gentamicin
Netilmicin had appreciably greater activity against gram-negative organisms that were resistant to gentamicin because these species synthesized aminoglycoside 3-N-acetyltransferase I or aminglycoside 2″-O-nucleotidyl transferase.
Comparison of Activity of Sisomicin and Gentamicin in Mouse Protection Tests with Gram-Negative Bacilli
The efficacy of sisomicin and gentamicin was compared in mouse protection studies against strains of Escherichia coli, Klebsiella sp., Enterobacter aerogenes, Serratia marcescens, Proteus mirabilis,


Tobramycin: In Vitro Activity and Comparison with Kanamycin and Gentamicin
Tobramycin was found to have satisfactory in vitro activity against many clinically important organisms, including strains resistant to gentamicin and kanamycin.
Tobramycin (Nebramycin Factor 6): In Vitro Activity Against Pseudomonas aeruginosa
Tobramycin appears to be a potent, rapidly bactericidal antibiotic against P. aeruginosa and merits clinical evaluation.
Evaluation of the in vitro activity of tobramycin as compared with that of gentamicin sulfate.
The in vitro activity of tobramycin was quantitatively compared with that of gentamicin sulfate against 195 bacterial isolates from clinical material and on the basis of the obtained data, the following criteria are proposed for the interpretation of diffusion susceptibility tests with 10-mug discs of Gentamicin and tobramYcin.
Susceptibility of Pseudomonas aeruginosa to gentamicin sulfate in vitro: lack of correlation between disc diffusion and broth dilution sensitivity data.
It is recommended that those isolates of P. aeruginosa that yield zones of inhibition less than 12 mm in diameter be disc susceptibility-tested once more and should then be subjected to the broth dilution test before they are designated as sensitive or resistant to gentamicin.
Biological activity of the antibiotic components of the gentamicin complex
It is reported that submerged fermentations of M. purpurea NRRL 2953 produce a gentamicin complex consisting of three antibiotic components which have been designated as C1, Cl, and C2, and details of the isolation procedure and differential microbiological assay of the mixture will be published elsewhere.
Antibiotic 6640. 3. Biological studies with antibiotic 6640, a new broad-spectrum aminoglycoside antibiotic.
Results of comparative ataxia tests in cats are reported and Antibiotic 6640 has up to 5 times the activity of gentamicin in therapeutic and prophylactic antibacterial tests in mice.
Antibiotic 6640, a new Micromonospora-produced aminoglycoside antibiotic.
Antibiotic 6640 is a new aminoglycoside antibiotic isolated from fermentation broths of a new species of the genus Microm onospora, named Micromonospora inyoensis, which appears to be quite similar to that of gentamicin in vitro and in vivo.
Antibiotic susceptibility testing by a standardized single disk method.
Recommendations of the National Committee for Clinical Laboratory Standards continue to be based on this publication; the “Kirby-Bauer” method is, among the many disk methods used in other countries, still the one that has been researched most thoroughly and updated continuously.