In Situ C4 Phosphoenolpyruvate Carboxylase Activity and Kinetic Properties in Isolated Digitaria Sanguinalis Mesophyll Cells

Abstract

Isolated mesophyll cells from darkened leaves of the C4 plant Digitaria sanguinalis keep functional plasmodesmata that allow the free exchange of low molecular mass compounds with the surrounding medium. This cell suspension system has been used to measure C4 PEPC activity in situ using a spectrophotometric assay. Compared to the extracted enzyme assayed in… (More)
DOI: 10.1023/B:PRES.0000017179.31351.f0

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