In-Gel Protein Phosphatase Assay Using Fluorogenic Substrates.

  title={In-Gel Protein Phosphatase Assay Using Fluorogenic Substrates.},
  author={Isamu Kameshita and Noriyuki Sueyoshi and Atsuhiko Ishida},
  journal={Analytical biochemistry},
  volume={400 1},
Protein phosphorylation plays important roles in regulating a variety of biological processes in animals, plants, and microorganisms. Therefore, it is important to use appropriate techniques to detect and analyze protein kinases and protein phosphatases. In this chapter, we describe the method to detect protein phosphatase activities using fluorogenic… CONTINUE READING