In‐depth proteomic analysis of banana (Musa spp.) fruit with combinatorial peptide ligand libraries

@article{Esteve2013IndepthPA,
  title={In‐depth proteomic analysis of banana (Musa spp.) fruit with combinatorial peptide ligand libraries},
  author={Clara Esteve and Alfonsina D'Amato and Mar{\'i}a Luisa Marina and Mar{\'i}a Concepci{\'o}n Garc{\'i}a and Pier Giorgio Righetti},
  journal={ELECTROPHORESIS},
  year={2013},
  volume={34}
}
Musa ssp. is among the world's leading fruit crops. Although a strong interest on banana biochemistry exists in the scientific community, focused on metabolite composition, proteins have been scarcely investigated even if they play an important role in food allergy and stability, are a source of biologically active peptides, and can provide information about nutritional aspects of this fruit. In this work we have employed the combinatorial peptide ligand libraries after different types of… Expand
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References

SHOWING 1-10 OF 42 REFERENCES
Identification of avocado (Persea americana) pulp proteins by nano‐LC‐MS/MS via combinatorial peptide ligand libraries
TLDR
The proteome of avocado pulp was explored in depth by extracting proteins with capture by combinatorial peptide ligand libraries under conditions mimicking reverse‐phase capture at pH 2.2 and it was possible to identify the already known avocado allergen Pers a 1 and different proteins susceptible to be allergens. Expand
Identification of olive (Olea europaea) seed and pulp proteins by nLC-MS/MS via combinatorial peptide ligand libraries.
TLDR
The allergenic thaumatin-like protein (Ole e 13) was confirmed, among the other 231, as the most abundant protein in the olive pulp, and the overall investigation up to the present of the olive proteome was concluded. Expand
Molecular and immunological characterization of Mus a 5 allergen from banana fruit.
TLDR
Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy and upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner. Expand
Purification and structural analysis of an abundant thaumatin-like protein from ripe banana fruit
TLDR
It is demonstrated for the first time that fruit-specific (abundant) TLPs are not confined to dicots but occur also in fruits of monocot species. Expand
Functional proteome analysis of the banana plant (Musa spp.) using de novo sequence analysis of derivatized peptides.
TLDR
Using three frequently used homology-based search algorithms, the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence analysis was able to identify more than 40 proteins from banana, a nonmodel plant with unsequenced genome. Expand
A critical evaluation of sample extraction techniques for enhanced proteomic analysis of recalcitrant plant tissues
TLDR
The results suggest that the Phe protocol is highly effective with more recalcitrant tissues and that a combination of TCA‐A and Phe methods provides enhanced 2‐DE based proteomic analyses of most plant tissues. Expand
Mehercules, adhuc Bacchus! The debate on wine proteomics continues.
TLDR
The detection of so many low-abundance species suggests that proteomic (coupled to peptidomic) data might be used for typing high-quality products (grand crus) to assess their genuineness and protect them from fraudulent imitations. Expand
A workflow for peptide-based proteomics in a poorly sequenced plant: a case study on the plasma membrane proteome of banana.
TLDR
By applying a workflow for membrane proteomics in banana, a poorly sequenced plant, integral plasma membrane proteins from banana leaves were successfully identified. Expand
Resolution of the structure of the allergenic and antifungal banana fruit thaumatin-like protein at 1.7-A.
TLDR
The structure of a thaumatin-like protein from banana (Musa acuminata) fruit, an allergen with antifungal properties, was solved at 1.7-A-resolution, by X-ray crystallography and revealed the presence of conserved residues of exposed epitopic determinants that are presumably responsible for the allergenic properties of banana fruit towards susceptible individuals. Expand
Banana (Musa spp.) as a model to study the meristem proteome: Acclimation to osmotic stress
TLDR
The results suggest that the maintenance of an osmoprotective intracellular sucrose concentration, the enhanced expression of particular genes of the energy‐conserving glycolysis and the conservation of the cell wall integrity are essential to maintain homeostasis, to acclimate and to survive dehydration. Expand
...
1
2
3
4
5
...