In‐depth proteomic analysis of banana (Musa spp.) fruit with combinatorial peptide ligand libraries

@article{Esteve2013IndepthPA,
  title={In‐depth proteomic analysis of banana (Musa spp.) fruit with combinatorial peptide ligand libraries},
  author={Clara Esteve and Alfonsina D’Amato and Mar{\'i}a Luisa Marina and Mar{\'i}a Concepci{\'o}n Garc{\'i}a and Pier Giorgio Righetti},
  journal={ELECTROPHORESIS},
  year={2013},
  volume={34}
}
Musa ssp. is among the world's leading fruit crops. Although a strong interest on banana biochemistry exists in the scientific community, focused on metabolite composition, proteins have been scarcely investigated even if they play an important role in food allergy and stability, are a source of biologically active peptides, and can provide information about nutritional aspects of this fruit. In this work we have employed the combinatorial peptide ligand libraries after different types of… 
View on Wiley
ncbi.nlm.nih.gov
45 Citations
Background Citations
5
Methods Citations
5

45 Citations

A sarabande of tropical fruit proteomics: Avocado, banana, and mango

TLDR
The present review highlights the progress made in plant proteomics via the introduction of combinatorial peptide ligand libraries (CPLL) for detecting low‐abundance species and reports on a trio of tropical fruits, namely, banana, avocado, and mango.

Proteome investigation of the non-model plant pomegranate (Punica granatum L.)

TLDR
A gel-free, shotgun proteomics approach was used to characterize pomegranate aril proteome by nanoliquid chromatography–high-resolution tandem mass spectrometry, the first work aimed at the proteomic characterization of Punica granatum.

Identification of plum and peach seed proteins by nLC-MS/MS via combinatorial peptide ligand libraries.

The Plantain Proteome, a Focus on Allele Specific Proteins Obtained from Plantain Fruits

TLDR
The aim of this work was to characterize the fruit proteome of common dessert bananas and plantains and to identify proteins that are only encoded by the plantain genome, and found 37 alleles to be unique for plantain covered by 59 peptides.

Global proteome analysis in plants by means of peptide libraries and applications.

Novel low-abundance allergens from mango via combinatorial peptide libraries treatment: A proteomics study.

Making progress in plant proteomics for improved food safety

Low-abundance plant protein enrichment with peptide libraries to enlarge proteome coverage and related applications.

The “Dark Side” of Food Stuff Proteomics: The CPLL-Marshals Investigate

TLDR
The present review deals with analysis of the proteome of animal and plant-derived food stuff, as well as of non-alcoholic and alcoholic beverages, with the aim of tracing the industrial manipulations and herbal usage prior to their commercialization.

Proteomic and metabolomic study of wax apple (Syzygium samarangense) fruit during ripening process

TLDR
This is the first study investigating the changes in the proteins and metabolites in wax apple at different developmental stages and the information obtained will be helpful in developing biomarkers for breeders and help the plant researchers to avoid wax apple cultivation problems such as fruit cracking.

References

SHOWING 1-10 OF 42 REFERENCES

Identification of avocado (Persea americana) pulp proteins by nano‐LC‐MS/MS via combinatorial peptide ligand libraries

TLDR
The proteome of avocado pulp was explored in depth by extracting proteins with capture by combinatorial peptide ligand libraries under conditions mimicking reverse‐phase capture at pH 2.2 and it was possible to identify the already known avocado allergen Pers a 1 and different proteins susceptible to be allergens.

Identification of olive (Olea europaea) seed and pulp proteins by nLC-MS/MS via combinatorial peptide ligand libraries.

Molecular and immunological characterization of Mus a 5 allergen from banana fruit.

TLDR
Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy and upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner.

Purification and structural analysis of an abundant thaumatin-like protein from ripe banana fruit

TLDR
It is demonstrated for the first time that fruit-specific (abundant) TLPs are not confined to dicots but occur also in fruits of monocot species.

Functional proteome analysis of the banana plant (Musa spp.) using de novo sequence analysis of derivatized peptides.

TLDR
Using three frequently used homology-based search algorithms, the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence analysis was able to identify more than 40 proteins from banana, a nonmodel plant with unsequenced genome.

A critical evaluation of sample extraction techniques for enhanced proteomic analysis of recalcitrant plant tissues

TLDR
The results suggest that the Phe protocol is highly effective with more recalcitrant tissues and that a combination of TCA‐A and Phe methods provides enhanced 2‐DE based proteomic analyses of most plant tissues.

Mehercules, adhuc Bacchus! The debate on wine proteomics continues.

TLDR
The detection of so many low-abundance species suggests that proteomic (coupled to peptidomic) data might be used for typing high-quality products (grand crus) to assess their genuineness and protect them from fraudulent imitations.

A workflow for peptide-based proteomics in a poorly sequenced plant: a case study on the plasma membrane proteome of banana.

Resolution of the structure of the allergenic and antifungal banana fruit thaumatin-like protein at 1.7-A.

Banana (Musa spp.) as a model to study the meristem proteome: Acclimation to osmotic stress

TLDR
The results suggest that the maintenance of an osmoprotective intracellular sucrose concentration, the enhanced expression of particular genes of the energy‐conserving glycolysis and the conservation of the cell wall integrity are essential to maintain homeostasis, to acclimate and to survive dehydration.