In vitro production of Indian citrus ringspot virus (ICRSV) free Kinnow plants employing thermotherapy coupled with shoot tip grafting

@article{Sharma2007InVP,
  title={In vitro production of Indian citrus ringspot virus (ICRSV) free Kinnow plants employing thermotherapy coupled with shoot tip grafting},
  author={Sanjeev Sharma and Balwinder Singh and G. Prasanna Rani and Aijaz Asghar Zaidi and Vipin Hallan and Avinash Kaur Nagpal and Gurdeep Singh Virk},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2007},
  volume={92},
  pages={85-92}
}
Indian citrus ringspot virus (ICRSV) is known to cause serious disease problem in Kinnow (Citrus nobilis Lour × C. deliciosa Tenora) plants. This work reports the elimination of ICRSV by using thermotherapy coupled with shoot tip grafting in vitro. Nodal segments from infected mother plants (indexed by indirect ELISA and RT-PCR) were treated both in water bath and moist hot air at different temperatures viz. 40, 45 and 50°C for 30, 60 and 120 min and cultured on MS medium containing 2-iP (1 mg… CONTINUE READING
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