In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line

@article{Deyrieux2007InVC,
  title={In vitro culture conditions to study keratinocyte differentiation using the HaCaT cell line},
  author={Adeline F. Deyrieux and Victor Gideon Wilson},
  journal={Cytotechnology},
  year={2007},
  volume={54},
  pages={77-83}
}
In vitro models to study the process of keratinocyte differentiation have been hindered by the stringent culture requirements and limitations imposed by the inherent properties of the cells. Primary keratinocytes only have a finite life span, while transformed cell lines exhibit many phenotypic features not found in normal cells. The spontaneously immortalized HaCaT cell line has been a widely employed keratinocyte model due to its ease of propagation and near normal phenotype, but protocols… CONTINUE READING
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