Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references.

@article{Ghirlando2013ImprovingTT,
  title={Improving the thermal, radial, and temporal accuracy of the analytical ultracentrifuge through external references.},
  author={Rodolfo Ghirlando and Andrea Balbo and Grzegorz Piszczek and Patrick H. Brown and Marc S. Lewis and Chad A. Brautigam and Peter Schuck and Huaying Zhao},
  journal={Analytical biochemistry},
  year={2013},
  volume={440 1},
  pages={81-95}
}
Sedimentation velocity (SV) is a method based on first principles that provides a precise hydrodynamic characterization of macromolecules in solution. Due to recent improvements in data analysis, the accuracy of experimental SV data emerges as a limiting factor in its interpretation. Our goal was to unravel the sources of experimental error and develop improved calibration procedures. We implemented the use of a Thermochron iButton temperature logger to directly measure the temperature of a… CONTINUE READING