Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns.

@article{Girault1989ImprovingTQ,
  title={Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns.},
  author={Jean Antoine Girault and Fred S. Gorelick and Paul Greengard},
  journal={Analytical biochemistry},
  year={1989},
  volume={182 1},
  pages={193-6}
}
Unwanted reactivity of polyclonal antisera against keratins ("fingerprint proteins") is a problem commonly encountered when proteins transferred to nitrocellulose are studied by immunoblotting. Immunoreactivity against keratins is generally accompanied by a spotted background. This antikeratin immunoreactivity could be removed by adsorption of the antisera to human keratin bound to nitrocellulose. Larger amounts of antisera were purified from contaminant antikeratin antibodies by a single… CONTINUE READING

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