Improving the nutritional value of Golden Rice through increased pro-vitamin A content

@article{Paine2005ImprovingTN,
  title={Improving the nutritional value of Golden Rice through increased pro-vitamin A content},
  author={J. A. Paine and C. Shipton and S. Chaggar and R. Howells and Mike J Kennedy and G. Vernon and S. Wright and E. Hinchliffe and Jessica L Adams and A. Silverstone and R. Drake},
  journal={Nature Biotechnology},
  year={2005},
  volume={23},
  pages={482-487}
}
'Golden Rice' is a variety of rice engineered to produce β-carotene (pro-vitamin A) to help combat vitamin A deficiency, and it has been predicted that its contribution to alleviating vitamin A deficiency would be substantially improved through even higher β-carotene content. We hypothesized that the daffodil gene encoding phytoene synthase (psy), one of the two genes used to develop Golden Rice, was the limiting step in β-carotene accumulation. Through systematic testing of other plant psys, we identified a psy from maize that substantially increased carotenoid accumulation in a model plant system. [...] Key Method We went on to develop 'Golden Rice 2' introducing this psy in combination with the Erwinia uredovora carotene desaturase (crtI) used to generate the original Golden Rice. We observed an increase in total carotenoids of up to 23-fold (maximum 37 μg/g) compared to the original Golden Rice and a preferential accumulation of β-carotene.Expand

Paper Mentions

Interventional Clinical Trial
The main objective of this human trial is the demonstration that β-carotene in fortified maize has good bioavailability as a plant source of vitamin A and that when humans ingest the… Expand
ConditionsHealthy
InterventionOther
High Beta-Carotene Rice in Asia: Techniques and Implications
TLDR
In this chapter, an attempt was made to discuss the details of the techniques and implications of high beta-carotene in rice on Asian context. Expand
Golden Rice is an effective source of vitamin A.
TLDR
Beta-carotene derived from Golden Rice is effectively converted to vitamin A in humans, and this trial was registered as NCT00680355. Expand
Generation of transgenic maize with enhanced provitamin A content
TLDR
Gene expression analyses suggest that increased accumulation of β-carotene is due to an up-regulation of the endogenous lycopene β-cylase, and set the stage for the design of transgenic approaches to generate provitamin A-rich maize that will help alleviate VAD. Expand
Potential of golden potatoes to improve vitamin A and vitamin E status in developing countries
TLDR
It is suggested that a 150 g serving of boiled golden potatoes has the potential to contribute 42% and 23% of the daily requirement of retinol activity equivalents (RAE), as well as 34 and 17% ofThe daily vitamin E requirement for children and women of reproductive age, respectively. Expand
Effect of codon optimization on the enhancement of the β-carotene contents in rice endosperm
TLDR
The codon optimization of the transgene might be an effective tool in genetic engineering for crop improvement as proven at the enhanced levels of translation and carotenoid production. Expand
Metabolic Regulation of Carotenoid-Enriched Golden Rice Line
TLDR
The present study highlights the metabolic adaptation process of a transgenic golden rice line (homozygous T4 progeny of SKBR-244) after enhancing carotenoid biosynthesis, which would be helpful in the development of crops enriched inCarotenoids by expressing metabolic flux of pyruvate biosynthesis. Expand
Alteration of Carotenoid Metabolic Machinery by β-Carotene Biofortification in Rice Grains
TLDR
Results showed that transgene-driven biofortification of carotenoids was made and maintained over several transgenic generations with a possible negative feed-back control of endogenousCarotenoid metabolism during seed development. Expand
Why Is Golden Rice Golden (Yellow) Instead of Red?1[w]
TLDR
The transgenic phenotype is not due to up-regulation of expression of the endogenous rice pathway in response to the transgenes, as was suggested to be the case in tomato (Lycopersicon esculentum) fruit, where CRTI expression resulted in a similar carotenoid phenomenon. Expand
Metabolic Engineering of Potato Carotenoid Content through Tuber-Specific Overexpression of a Bacterial Mini-Pathway
TLDR
This is the highest carotenoid and beta-carotene content reported for biofortified potato as well as for any of the four major staple foods (the next best event being “Golden Rice 2”, with 31 mcg/g dry weight beta- carotene). Expand
Single genetic locus improvement of iron, zinc and β-carotene content in rice grains
TLDR
The results establish a proof-of-concept for multi-nutrient enrichment of rice grains from a single genetic locus, thus offering a sustainable and effective approach to address different micronutrient deficiencies at once. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 39 REFERENCES
Elevation of the provitamin A content of transgenic tomato plants
TLDR
Transgenic lines containing a bacterial carotenoid gene (crtI) encoding the enzyme phy toene desaturase, which converts phytoene into lycopene are produced, finding the phenotype has been found to be stable and reproducible over at least four generations. Expand
Bioengineered 'golden' indica rice cultivars with beta-carotene metabolism in the endosperm with hygromycin and mannose selection systems.
TLDR
This is the first report of using nonantibiotic pmi driven by a novel promoter in generating transgenic indica rice for possible future use in human nutrition. Expand
Golden rice: what role could it play in alleviation of vitamin A deficiency?
TLDR
Golden rice (GR) is a new rice variety that has been genetically modified to contain beta-carotene, a source of vA, which was undertaken as a strategy to address VAD, which is widespread in less developed countries of Asia. Expand
Seed-specific overexpression of phytoene synthase: increase in carotenoids and other metabolic effects
A bacterial phytoene synthase (crtB) gene was overexpressed in a seed-specific manner and the protein product targeted to the plastid in Brassica napus (canola). The resultant embryos from theseExpand
Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner
  • P. Fraser, S. Romer, +6 authors P. Bramley
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2002
TLDR
Metabolic control analysis suggests that the presence of an additional phytoene synthase reduces the regulatory effect of this step over the carotenoid pathway, and the activities of other enzymes in the pathway (isopentenyl diphosphate isomerase, geranylgeranyl diph phosphate synthase, and incorporation of isopentanyl dphosphate into phy toene) were not significantly altered. Expand
Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis.
TLDR
It is demonstrated for the first time that it is in principle possible to engineer a critical step in provitamin A biosynthesis in a non-photosynthetic, carotenoid-lacking plant tissue. Expand
Carotenoid Biosynthesis during Tomato Fruit Development (Evidence for Tissue-Specific Gene Expression)
TLDR
The results suggest that transcription of Psy and Pds is regulated developmentally, with expression being considerably elevated in chromoplast-containing tissues, and lends support to the hypothesis of divergent genes encoding these enzymes. Expand
Functional expression of the Erwinia uredovora carotenoid biosynthesis gene crtl in transgenic plants showing an increase of beta-carotene biosynthesis activity and resistance to the bleaching herbicide norflurazon.
TLDR
Immunogold localization showed that the location of the gene product Crtl was preferentially in the thylakoids, and the transgenic tobacco acquired elevated resistance to the bleaching herbicide norflurazon. Expand
Potential health benefits of Golden Rice: a Philippine case study
Abstract Golden Rice has been genetically modified to produce beta-carotene in the endosperm of grain. It could improve the vitamin A status of deficient food consumers, especially women and childrenExpand
A tomato gene expressed during fruit ripening encodes an enzyme of the carotenoid biosynthesis pathway.
TLDR
It is shown that a tomato cDNA for a gene (Psy1) expressed during fruit ripening directs the in vitro synthesis of a 47-kDa protein which, upon import into isolated chloroplasts, is processed to a mature 42- kDa form. Expand
...
1
2
3
4
...