Improving the nutritional value of Golden Rice through increased pro-vitamin A content

@article{Paine2005ImprovingTN,
  title={Improving the nutritional value of Golden Rice through increased pro-vitamin A content},
  author={J. A. Paine and C. A. Shipton and S. Chaggar and R. Howells and M. Kennedy and Gareth Vernon and S. Wright and E. Hinchliffe and J. Adams and A. Silverstone and R. Drake},
  journal={Nature Biotechnology},
  year={2005},
  volume={23},
  pages={482-487}
}
  • J. A. Paine, C. A. Shipton, +8 authors R. Drake
  • Published 2005
  • Biology, Medicine
  • Nature Biotechnology
  • 'Golden Rice' is a variety of rice engineered to produce β-carotene (pro-vitamin A) to help combat vitamin A deficiency, and it has been predicted that its contribution to alleviating vitamin A deficiency would be substantially improved through even higher β-carotene content. We hypothesized that the daffodil gene encoding phytoene synthase (psy), one of the two genes used to develop Golden Rice, was the limiting step in β-carotene accumulation. Through systematic testing of other plant psys, we identified a psy from maize that substantially increased carotenoid accumulation in a model plant system. [...] Key Method We went on to develop 'Golden Rice 2' introducing this psy in combination with the Erwinia uredovora carotene desaturase (crtI) used to generate the original Golden Rice. We observed an increase in total carotenoids of up to 23-fold (maximum 37 μg/g) compared to the original Golden Rice and a preferential accumulation of β-carotene.Expand Abstract
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    Paper Mentions

    INTERVENTIONAL CLINICAL TRIAL
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    ConditionsHealthy
    InterventionOther
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    References

    SHOWING 1-10 OF 39 REFERENCES
    Elevation of the provitamin A content of transgenic tomato plants
    • 394
    Seed-specific overexpression of phytoene synthase: increase in carotenoids and other metabolic effects
    • 476
    Evaluation of transgenic tomato plants expressing an additional phytoene synthase in a fruit-specific manner
    • P. Fraser, S. Romer, +6 authors P. Bramley
    • Medicine, Biology
    • Proceedings of the National Academy of Sciences of the United States of America
    • 2002
    • 413
    • PDF