• Corpus ID: 53594458

Improving the health benefits of broccoli through myrosinase maintenance

@inproceedings{Dosz2014ImprovingTH,
  title={Improving the health benefits of broccoli through myrosinase maintenance},
  author={Edward B. Dosz},
  year={2014}
}
Sulforaphane is produced from the hydrolysis of the glucosinolate glucoraphanin in the presence of the endogenous enzyme myrosinase. Sulforaphane has been shown to provide cancer prevention through a number of mechanisms including the upregulation of detoxification enzymes and epigenetic changes. Optimal temperature and pH for sulforaphane formation from broccoli was determined. Sulforaphane formation was measured in three commercially frozen broccoli samples preand post-cooking. The results… 
ideals.illinois.edu
1 Citations
Background Citations
1

One Citation

GREEN SYNTHESIS, CHARACTERIZATION AND BIOLOGICAL EVALUATION OF ZINC NANOPARTICLES FROM FLOWER EXTRACT OF BRASSICA OLERACEA VAR.??? ITALICA
The current work focused on green synthesis of Zinc nanoparticles (ZnNPs) using the aqueous extract of Brassica oleracea italica inflorescence acting as a reducing agent. The characterization of…

References

SHOWING 1-10 OF 26 REFERENCES
The activity of myrosinase from broccoli (Brassica oleracea L. cv. Italica): influence of intrinsic and extrinsic factors.
TLDR
Investigation of the potential of some intrinsic and extrinsic factors for controlling/altering activity of myrosinase from broccoli found a combination of MgCl2 and ascorbic acid was found to enhance enzyme activity, which could be the basis for controlling glucosinolate hydrolysis in food preparation and processing.
Protection of Humans by Plant Glucosinolates: Efficiency of Conversion of Glucosinolates to Isothiocyanates by the Gastrointestinal Microflora
TLDR
Although no demographic factors affecting conversion efficiency have been identified, there are clearly diurnal variations: conversion of GS to DTC was greater during the day, but conversion of ITC to ITC was more efficient at night.
Enhancing sulforaphane absorption and excretion in healthy men through the combined consumption of fresh broccoli sprouts and a glucoraphanin-rich powder
TLDR
Combining broccoli sprouts with the GRP powder synergistically enhanced the early appearance of SF, offering insight into the combination of foods for improved health benefits of foods that reduce the risk for cancer.
THE MYROSINASE-GLUCOSINOLATE SYSTEM, ITS ORGANISATION AND BIOCHEMISTRY
TLDR
The compartmentalization of the components of the myrosinase-glucosinolate system and the cell-specific expression of theMyrosinases represents a unique plant defence system.
Kinetics of the stability of broccoli (Brassica oleracea Cv. Italica) myrosinase and isothiocyanates in broccoli juice during pressure/temperature treatments.
TLDR
The Brassicaceae plant family contains high concentrations of glucosinolates, which can be hydrolyzed by myrosinase yielding products having an anticarcinogenic activity, and a mathematical model describing the pressure and temperature dependence of myros inactivation rate constants was constructed.
Food matrix effects on bioactivity of broccoli-derived sulforaphane in liver and colon of F344 rats.
TLDR
All diets were substantially more effective in the colon than in the liver and that broccoli-GS was more potent than SF or broccoli-HP and that urinary SF-mercapturic acid correlated with QR activity, not with dietary intake.
Temperature and pressure stability of mustard seed (Sinapis alba L.) myrosinase
Revised determination of free and complexed myrosinase activities in plant extracts.
Comparative biochemical characterization of nitrile‐forming proteins from plants and insects that alter myrosinase‐catalysed hydrolysis of glucosinolates
TLDR
Both epithiospecifier protein and nitrile‐specifier protein appear to be true enzymes rather than allosteric cofactors of myrosinases, based on their substrate and product specificities and the fact that the proportion of glucosinolates hydrolysed to nitriles does not remain constant whenMyrosinase activity varies.
Bioavailability and inter-conversion of sulforaphane and erucin in human subjects consuming broccoli sprouts or broccoli supplement in a cross-over study design.
...
...