Improving the decolorization for textile dyes of a metagenome-derived alkaline laccase by directed evolution

@article{Liu2011ImprovingTD,
  title={Improving the decolorization for textile dyes of a metagenome-derived alkaline laccase by directed evolution},
  author={Yuhuan Liu and Mao Ye and Yi Lu and Xia Zhang and Gang Li},
  journal={Applied Microbiology and Biotechnology},
  year={2011},
  volume={91},
  pages={667-675}
}
To obtain better performing laccases for textile dyes decolorization, random mutagenesis of Lac591, a metagenome-derived alkaline laccase, was carried out. After three rounds of error-prone PCR and high-throughput screening by assaying enzymatic activity toward the phenolic substrate 2,6-dimethoxyphenol (2,6-DMP), a mutant (Lac3T93) with remarkably improved enzymatic activity was obtained. Sequence analysis revealed that four amino acid substitutions (N40S, V55A, F62L, and E316V) were… CONTINUE READING