Improving stable transfection efficiency: antioxidants dramatically improve the outgrowth of clones under dominant marker selection.

@article{Brielmeier1998ImprovingST,
  title={Improving stable transfection efficiency: antioxidants dramatically improve the outgrowth of clones under dominant marker selection.},
  author={Markus Brielmeier and J M B{\'e}chet and Melanie Falk and Michael Pawlita and Axel Polack and Georg W. Bornkamm},
  journal={Nucleic acids research},
  year={1998},
  volume={26 9},
  pages={2082-5}
}
Many cell lines are sensitive to growth at low cell density and undergo apoptosis induced by oxidative stress if the cell density is decreased below a critical threshold. In stable transfection experiments this cell density-dependent growth may be the limiting factor, since during drug selection the cell density falls below the critical threshold, precluding outgrowth of transfected clones. We describe here a simple protocol for the establishment of stably transfected human B cell lines making… CONTINUE READING
24 Citations
12 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 24 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 12 references

Int. J. Cancer

  • M H Falk, T Meier, R D Issels, M Brielmeier, B Scheffer, G W Bornkamm
  • Int. J. Cancer
  • 1998

BioTechniques

  • C Baum, P Forster, Hegewisch Becker, S Harbers
  • BioTechniques
  • 1994

Proc. Natl. Acad. Sci. USA, 91

  • M Mayer, M Noble
  • Proc. Natl. Acad. Sci. USA, 91
  • 1993

Nature

  • C D Gregory, C Dive, +4 authors A B Rickinson
  • Nature
  • 1991

Cell Gene

  • V Steimle, L A Otten, M Zufferey, B Mach
  • Cell Gene
  • 1990

Neuron

  • T H Murphy, M Miyamoto, A Sastre, R L Schnaar, J T Coyle
  • Neuron
  • 1989

Anal. Biochem

  • J C Knutson, D Yee
  • Anal. Biochem
  • 1987

Nucleic Acids Res

  • G Chu, H Hayakawa, P Berg
  • Nucleic Acids Res
  • 1987

Mol. Cell. Biol

  • F Toneguzzo, A C Hayday, A Keating
  • Mol. Cell. Biol
  • 1986

Toxicol. Lett

  • U Andrae, J Singh, K Ziegler-Skylakakis
  • Toxicol. Lett
  • 1985

Similar Papers

Loading similar papers…