Improving size-exclusion chromatography separation for glycogen.

  title={Improving size-exclusion chromatography separation for glycogen.},
  author={Mitchell A. Sullivan and Prudence O. Powell and Torsten Witt and Francisco Vilaplana and Eugeni Roura and Robert G Gilbert},
  journal={Journal of chromatography. A},
Glycogen is a hyperbranched glucose polymer comprised of glycogen β particles, which can also form much larger composite α particles. The recent discovery using size-exclusion chromatography (SEC) that fewer, smaller, α particles are found in diabetic-mouse liver compared to healthy mice highlights the need to achieve greater accuracy in the size separation methods used to analyze α and β particles. While past studies have used dimethyl sulfoxide as the SEC eluent to analyze the molecular size… CONTINUE READING


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