Improving protein secretion of a transglutaminase-secreting Corynebacterium glutamicum recombinant strain on the basis of 13C metabolic flux analysis.

@article{Umakoshi2011ImprovingPS,
  title={Improving protein secretion of a transglutaminase-secreting Corynebacterium glutamicum recombinant strain on the basis of 13C metabolic flux analysis.},
  author={Motoki Umakoshi and Takashi Hirasawa and Chikara Furusawa and Yasuhiro Takenaka and Yoshimi Kikuchi and Hiroshi Shimizu},
  journal={Journal of bioscience and bioengineering},
  year={2011},
  volume={112 6},
  pages={595-601}
}
Corynebacterium glutamicum is known as a host species for amino acid production. This microorganism was recently noticed as a host that produces secreted proteins. In this study, we performed (13)C metabolic flux analysis ((13)C-MFA) on a recombinant C. glutamicum strain that secretes a heterologous transglutaminase (TGase) to improve TGase secretion. For the (13)C-MFA of a TGase-secreting C. glutamicum strain in batch cultivation, a (13)C-labeling experiment and measurement of mass isotopomer… CONTINUE READING