Improving molecular detection of fungal DNA in formalin-fixed paraffin-embedded tissues: comparison of five tissue DNA extraction methods using panfungal PCR.

@article{MuozCadavid2010ImprovingMD,
  title={Improving molecular detection of fungal DNA in formalin-fixed paraffin-embedded tissues: comparison of five tissue DNA extraction methods using panfungal PCR.},
  author={C Mu{\~n}oz-Cadavid and Stephen Rudd and Sherif R. Zaki and Mayank Patel and Stephen A. Moser and Mary Eleanor Brandt and Beatriz L G{\'o}mez},
  journal={Journal of clinical microbiology},
  year={2010},
  volume={48 6},
  pages={2147-53}
}
DNA extraction from formalin-fixed paraffin-embedded (FFPE) tissues is difficult and requires special protocols in order to extract small amounts of DNA suitable for amplification. Most described methods report an amplification success rate between 60 and 80%; therefore, there is a need to improve molecular detection and identification of fungi in FFPE tissue. Eighty-one archived FFPE tissues with a positive Gomori methenamine silver (GMS) stain were evaluated using five different commercial… CONTINUE READING

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