Improving molecular detection of Candida DNA in whole blood: comparison of seven fungal DNA extraction protocols using real-time PCR.

@article{Metwally2008ImprovingMD,
  title={Improving molecular detection of Candida DNA in whole blood: comparison of seven fungal DNA extraction protocols using real-time PCR.},
  author={Lobna A Metwally and Derek James Fairley and Peter Valentine Coyle and R J Hay and Sarah Hedderwick and Bethany McCloskey and Hugh J O'Neill and C. C. Webb and Wesam Elbaz and Ronan McMullan},
  journal={Journal of medical microbiology},
  year={2008},
  volume={57 Pt 3},
  pages={296-303}
}
The limitations of classical diagnostic methods for invasive Candida infections have led to the development of molecular techniques such as real-time PCR to improve diagnosis. However, the detection of low titres of Candida DNA in blood from patients with candidaemia requires the use of extraction methods that efficiently lyse yeast cells and recover small amounts of DNA suitable for amplification. In this study, a Candida-specific real-time PCR assay was used to detect Candida albicans DNA in… CONTINUE READING

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