Improving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double-bond reductase

@article{Lehka2017ImprovingHP,
  title={Improving heterologous production of phenylpropanoids in Saccharomyces cerevisiae by tackling an unwanted side reaction of Tsc13, an endogenous double-bond reductase},
  author={B. J. Lehka and Michael Eichenberger and Walden E Bj{\o}rn-Yoshimoto and Katherina Garc{\'i}a Vanegas and Nicolaas A A Buijs and N. B. Jensen and Jane D. Dyekj{\ae}r and H. Jenssen and E. Sim{\'o}n and Michael Naesby},
  journal={FEMS Yeast Research},
  year={2017},
  volume={17}
}
Abstract Phenylpropanoids, such as flavonoids and stilbenoids, are of great commercial interest, and their production in Saccharomyces cerevisiae is a very promising strategy. However, to achieve commercially viable production, each step of the process must be optimised. We looked at carbon loss, known to occur in the heterologous flavonoid pathway in yeast, and identified an endogenous enzyme, the enoyl reductase Tsc13, which turned out to be responsible for the accumulation of phloretic acid… Expand
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