Improving enantioselectivity towards tertiary alcohols using mutants of Bacillus sp. BP-7 esterase EstBP7 holding a rare GGG(X)-oxyanion hole

Abstract

Lipases and esterases are important biocatalysts for synthetic organic fine chemistry. An esterase from Bacillus sp. BP-7 (EstBP7) bears in its amino acid sequence a rare GGG(A)X oxyanion hole motif, where an uncommon threonine (T) is found at the third position. Detection of this pattern motivated evaluation of the ability of EstBP7 for conversion of… (More)
DOI: 10.1007/s00253-013-5458-9

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Cite this paper

@article{Fillat2013ImprovingET, title={Improving enantioselectivity towards tertiary alcohols using mutants of Bacillus sp. BP-7 esterase EstBP7 holding a rare GGG(X)-oxyanion hole}, author={Amanda Fillat and Pedro Romea and F{\`e}lix Urp{\'i} and F. Pastor and Pilar Diaz}, journal={Applied Microbiology and Biotechnology}, year={2013}, volume={98}, pages={4479-4490} }