Improving conjugation efficacy of Sorangium cellulosum by the addition of dual selection antibiotics

@article{Xia2008ImprovingCE,
  title={Improving conjugation efficacy of Sorangium cellulosum by the addition of dual selection antibiotics},
  author={Zhi-Jie Xia and Jing Wang and Wei Hu and Hong Di Liu and Xiu-Zhen Gao and Zhi-hong Wu and Peng-yi Zhang and Yue-Zhong Li},
  journal={Journal of Industrial Microbiology & Biotechnology},
  year={2008},
  volume={35},
  pages={1157-1163}
}
The conjugation protocols in myxobacterium Sorangium cellulosum are often inapplicable due to the strain-specific sensitivity to the presence of Escherichia coli cells or the resistances to many antibiotics. Here we report that the conjugative transfer of the mobilizable plasmid pCVD442 from E. coli DH5α (λ pir) to Sorangium strains could be greatly… CONTINUE READING