Corpus ID: 51681042

Improving Soluble Expression of Curcin-Transferrin Receptor-binding Peptide Fusion Protein with Glutathione Transferase-Small Ubiquitin-related Modifier System

@article{Jia2017ImprovingSE,
  title={Improving Soluble Expression of Curcin-Transferrin Receptor-binding Peptide Fusion Protein with Glutathione Transferase-Small Ubiquitin-related Modifier System},
  author={Changzeng Jia and S. Wang and Y. Chen and X. Xiao and Z. Su and Q. Zheng},
  journal={Journal of pharmaceutical and biomedical sciences},
  year={2017},
  volume={7}
}
  • Changzeng Jia, S. Wang, +3 authors Q. Zheng
  • Published 2017
  • Biology
  • Journal of pharmaceutical and biomedical sciences
    • Curcin can inhibit the proliferation of tumor cells and facilitate tumor cell apoptosis. In the current study, transferrin receptor (TFR)-binding peptide, TfRBP9, was fused with curcin and significantly enhanced the targeting of the anti-tumor ability of curcin. However, the recombinant curcin-TfRBP9 was expressed as an inclusion body in bacteria. The active recombinant curcin-TfRBP9 was obtained through dissolution, purification and renaturation. To achieve the soluble expression of… CONTINUE READING
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