Improving Saccharomyces cerevisiae ethanol production and tolerance via RNA polymerase II subunit Rpb7

@inproceedings{Qiu2017ImprovingSC,
  title={Improving Saccharomyces cerevisiae ethanol production and tolerance via RNA polymerase II subunit Rpb7},
  author={Zilong Qiu and Rongrong Jiang},
  booktitle={Biotechnology for biofuels},
  year={2017}
}
BACKGROUND Classical strain engineering methods often have limitations in altering multigenetic cellular phenotypes. Here we try to improve Saccharomyces cerevisiae ethanol tolerance and productivity by reprogramming its transcription profile through rewiring its key transcription component RNA polymerase II (RNAP II), which plays a central role in synthesizing mRNAs. This is the first report on using directed evolution method to engineer RNAP II to alter S. cerevisiae strain phenotypes… CONTINUE READING
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