Improving Performance and Operational Stability of Porcine Interferon-α Production by Pichia pastoris with Combinational Induction Strategy of Low Temperature and Methanol/Sorbitol Co-feeding

@article{Gao2015ImprovingPA,
  title={Improving Performance and Operational Stability of Porcine Interferon-α Production by Pichia pastoris with Combinational Induction Strategy of Low Temperature and Methanol/Sorbitol Co-feeding},
  author={Min-jie Gao and Xiaobei Zhan and Peng Gao and Xu Zhang and Shi-juan Dong and Zhen Li and Zhong-ping Shi and Chi-chung Lin},
  journal={Applied Biochemistry and Biotechnology},
  year={2015},
  volume={176},
  pages={493-504}
}
Various induction strategies were investigated for effective porcine interferon-α (pIFN-α) production by Pichia pastoris in a 10 L fermenter. We found that pIFN-α concentration could be significantly improved with the strategies of low-temperature induction or methanol/sorbitol co-feeding. On this basis, a combinational strategy of induction at lower temperature (20 °C) with methanol/sorbitol co-feeding has been proposed for improvement of pIFN-α production. The results reveal that maximal pIFN… CONTINUE READING

References

Publications referenced by this paper.
SHOWING 1-10 OF 33 REFERENCES