Improving N-glycosylation efficiency in Escherichia coli using shotgun proteomics, metabolic network analysis, and selective reaction monitoring.

@article{Pandhal2011ImprovingNE,
  title={Improving N-glycosylation efficiency in Escherichia coli using shotgun proteomics, metabolic network analysis, and selective reaction monitoring.},
  author={Jagroop Pandhal and Saw Yen Ow and Josselin Noirel and Phillip C. Wright},
  journal={Biotechnology and bioengineering},
  year={2011},
  volume={108 4},
  pages={902-12}
}
Recently, the prospect of using Escherichia coli as a host for human glycoprotein production has increased due to detailed characterization of the prokaryotic N-glycosylation process and the ability to transfer the system into this bacterium. Although functionality of the native Campylobacter jejuni N-glycosylation system in E. coli has been demonstrated, the efficiency of the process using the well-characterized C. jejuni glycoprotein AcrA, was found to be low at 13.4±0.9% of total extracted… CONTINUE READING

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