Improvements in transfection efficiency with chitosan modified poly(DL-lactide-co-glycolide) nanospheres prepared by the emulsion solvent diffusion method, for gene delivery.

@article{Tahara2011ImprovementsIT,
  title={Improvements in transfection efficiency with chitosan modified poly(DL-lactide-co-glycolide) nanospheres prepared by the emulsion solvent diffusion method, for gene delivery.},
  author={Kohei Tahara and Takeshi Sakai and Hiromitsu Yamamoto and Hirofumi Takeuchi and Naohide Hirashima and Yoshiaki Kawashima},
  journal={Chemical & pharmaceutical bulletin},
  year={2011},
  volume={59 3},
  pages={298-301}
}
This study sought to evaluate the in vitro transfection efficiency of plasmid DNA (pDNA)-loaded chitosan-modified poly(DL-lactide-co-glycolide) nanospheres (CS-PLGA NS) in a gene-delivery system. Using the emulsion solvent diffusion (ESD) method, pDNA-loaded PLGA NS was prepared and the surface of the PLGA NS was modified by binding to CS. Gene transfection ability of CS-PLGA NS was examined in A549 cells. The luciferase gene was used as a reporter gene. The pattern of luciferase activity by… CONTINUE READING
6 Citations
10 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-10 of 10 references

Eur. J. Pharm. Biopharm

  • K Tahara, H Yamamoto, N Hirashima, Y Kawashima
  • Eur. J. Pharm. Biopharm
  • 2010

Int. J. Pharm

  • K Tahara, T Sakai, H Yamamoto, H Takeuchi, N Hirashima, Y Kawashima
  • Int. J. Pharm
  • 2009

J. Pharm. Sci

  • C M Wiethoff, J G Koe, G S Koe, C R Middaugh
  • J. Pharm. Sci
  • 2004

Curr. Opin. Mol. Ther

  • H Büning, S A Nicklin, L Perabo, M Hallek, A H Baker
  • Curr. Opin. Mol. Ther
  • 2003

J. Biol. Chem

  • Rudolph C Plank, C Lausier, J Schillinger, U Müller, R H Rosenecker
  • J. Biol. Chem
  • 2003

Biochim. Biophys. Acta

  • T Ishii, Y Okahata, T Sato
  • Biochim. Biophys. Acta
  • 2001

Mol. Ther

  • A Maheshwari, R I Mahato, +4 authors S W Kim
  • Mol. Ther
  • 2000

J. Controlled Release

  • Wang D, Robinson D R Kwon, G S Samuel
  • J. Controlled Release
  • 1999

Adv. Drug Deliv. Rev

  • Smith J Zhang, Y Niven
  • Adv. Drug Deliv. Rev
  • 1997

J. Pharmacokinet. Biopharm

  • K Yamaoka, T Nakagawa, T Uno
  • J. Pharmacokinet. Biopharm
  • 1978

Similar Papers

Loading similar papers…