Corpus ID: 14234684

Improvement of tropane alkaloids production in hairy root cultures of Atropa belladonna by overexpressing pmt and h6h genes.

  title={Improvement of tropane alkaloids production in hairy root cultures of Atropa belladonna by overexpressing pmt and h6h genes.},
  author={Chunxian Yang and Min Chen and L. Zeng and L. Zhang and Xiaoqiang Liu and Xiaozhong Lan and K. Tang and Z. Liao},
  journal={Plant Omics},
Atropa belladonna L. is the commercial plant to produce tropane alkaloids including hyoscyamine and scopolamine, which are widely used as anticholinergic agents. In the present study, the genes encoding the key enzymes including putrescine N-methyltransferase (PMT) and hyoscyamine 6 �-hydroxylase (H6H) were simultaneously overexpressed in transgenic hairy root cultures of A. belladonna . All of the nine transgenic hairy root lines ovexpressing both pmt and h6h produced tropane alkaloids at… Expand
Genetic engineering of tropane alkaloid biosynthesis of hyoscyamus reticulatus L. hairy roots by pmt gene overexpression and feeding with putrescine
Abstract The use of biotechnology strategies to increase the production of natural products in medicinal plants, can be very useful and commercially profitable. In this study, control and transgenicExpand
Enhanced production of hyoscyamine and scopolamine from genetically transformed root culture of Hyoscyamus reticulatus L. elicited by iron oxide nanoparticles
It is suggested that FeNPs could be an effective elicitor in hairy root cultures in order to increase tropane alkaloid production. Expand
Improved tropane alkaloid production and changes in gene expression in hairy root cultures of two Hyoscyamus species elicited by silicon dioxide nanoparticles.
The effect of SiO2 NPs with two treatment times on the growth rate, total phenol and flavonoid content, antioxidant enzyme activity, tropane alkaloid yield and pmt and h6h gene expression levels in hairy roots of two Hyoscyamus species was investigated. Expand
Comparison of two hyoscyamine 6β-hydroxylases in engineering scopolamine biosynthesis in root cultures of Scopolia lurida.
This study provides a feasible way of overexpressing H6H to produce high-value scopolamine in engineered root cultures of S. lurida and found that HnH 6H was better than SlH6H for engineering scopolamines production. Expand
Raise up of Scopolamine in Hairy Roots Via Agrobacterium rhizogenes ATCC15834 as Compared with Untransformed Roots in Atropa komarovii
It was perceived that the scopolamine content in hairy roots was raised significantly compared to the control roots and it was evidenced that hairy roots gather a great number of metabolites that have a commercial significance. Expand
Effects of acetylsalicylic acid and UV-B on gene expression and tropane alkaloid biosynthesis in hairy root cultures of Anisodus luridus
All the four genes including PMT, TRI, CYP80F1 and H6H had a dramatic increase of gene expression that led to more accumulation of scopolamine and lower accumulation of hyoscyamine in hairy root cultures stressed under UV-B. Expand
Biotechnological Interventions of Hairy Roots of Tropane Alkaloid-Bearing Plants
The recent advances of pharmacological activity, hairy root, biosynthesis pathway, and genetic engineering were summarized, and problems along with prospects were also discussed. Expand
Silencing of quinolinic acid phosphoribosyl transferase (QPT) gene for enhanced production of scopolamine in hairy root culture of Duboisia leichhardtii
Scopolamine is a pharmaceutically important tropane alkaloid which is used therapeutically in the form of an anesthetic and antispasmodic drug. The present study demonstrates enhanced scopolamineExpand
Establishment of hairy root cultures and analysis of rotenoid in Tibetan medicinal plant Mirabilis himalaica
The highest hairy root induction rates were obtained using top leaves as explants, a preculture time of 2 d for young leaves, and Agrobacterium infection of young leaves for 20 min, confirming that the rol genes were integrated into the genome of M. himalaica. Expand
The novel paclitaxel-producing system: establishment of Corylus avellana L. hairy root culture
The investigations of explant type and different propagation media showed that the leafstalk was the most optimal explant for hairy root induction, and half-strength SH was the best culture medium for growth of the hairy roots in liquid medium. Expand


Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures.
  • L. Zhang, R. Ding, +11 authors K. Tang
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2004
It is concluded that transgenic plants Harboring both pmt and h6h possessed an increased flux in the tropane alkaloid biosynthetic pathway that enhanced scopolamine yield, which was more efficient than plants harboring only one of the two genes. Expand
Hairy root culture of Przewalskia tangutica for enhanced production of pharmaceutical tropane alkaloids
Hairy root cultures of P. tangutica were established to produce tropane alkaloids for the first time, and produced scopolamine and hyoscyamine at higher levels by comparison to the wild-type roots. Expand
Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine N-methyltransferase.
Putrescine N-methyltransferase (PMT) is the first alkaloid-specific enzyme for nicotine and tropane alkaloid formation. The pmt gene from Nicotiana tabacum was fused to the CaMV 35S promoter andExpand
Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus.
The results indicate that the same biosynthetic pathway in two related plant species can be differently regulated, and overexpression of a given gene does not necessarily lead to a similar accumulation pattern of secondary metabolites. Expand
Alkaloid production in Duboisia hybrid hairy roots and plants overexpressing the h6h gene
The h6h gene encoding H6H from Hyoscyamus niger, placed under the regulation of the CaMV 35S promoter, was introduced into the genome of a scopolamine-rich Duboisia hybrid by a binary vector system and the highest content ever reported for hairy roots of tropane-alkaloid-producing plants containing the 35S- h 6h transgene is reported. Expand
Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering
Results indicate that H. muticus strain Cairo has a potential for even more enhanced scopolamine production with more efficient gene-expression systems. Expand
Alkaloid production in Duboisia hybrid hairy root cultures overexpressing the pmt gene.
The N-methylputrescine levels of the resulting engineered hairy roots increased (2-4-fold) compared to wild type roots, but there was no significant increase in either tropane or pyridine-type alkaloids. Expand
Metabolic engineering of medicinal plants: transgenic Atropa belladonna with an improved alkaloid composition.
The hydroxylase gene from Hyoscyamus niger under the control of the cauliflower mosaic virus 35S promoter is introduced into hyoscyamine-rich Atropa belladonna by the use of an Agrobacterium-mediated transformation system and should prove useful as breeding materials for obtaining improved medicinal components. Expand
Putrescine N-Methyltransferase in Cultured Roots of Hyoscyamus albus: n-Butylamine as a Potent Inhibitor of the Transferase both in Vitro and in Vivo.
PMT was partially purified and characterized from cultured roots of Hyoscyamus albus that contain hyoscyamine as the main alkaloid and Monoamines were good competitive inhibitors of PMT, among which n-butylamine, cyclohexylamines, and exo-2-aminonorbornane were most inhibitory. Expand
Hyoscyamine 6 beta-hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is localized at the pericycle of the root.
Hyoscyamine 6 beta-hydroxylase (H6H; EC catalyzes the first reaction in the biosynthetic pathway from hyoscyamine to scopolamine in several solanaceous plants. Four monoclonal antibodiesExpand