Corpus ID: 14234684

Improvement of tropane alkaloids production in hairy root cultures of Atropa belladonna by overexpressing pmt and h6h genes.

@article{Yang2011ImprovementOT,
  title={Improvement of tropane alkaloids production in hairy root cultures of Atropa belladonna by overexpressing pmt and h6h genes.},
  author={Chunxian Yang and Min Chen and L. Zeng and L. Zhang and Xiaoqiang Liu and Xiaozhong Lan and K. Tang and Z. Liao},
  journal={Plant Omics},
  year={2011},
  volume={4},
  pages={29-33}
}
Atropa belladonna L. is the commercial plant to produce tropane alkaloids including hyoscyamine and scopolamine, which are widely used as anticholinergic agents. In the present study, the genes encoding the key enzymes including putrescine N-methyltransferase (PMT) and hyoscyamine 6 �-hydroxylase (H6H) were simultaneously overexpressed in transgenic hairy root cultures of A. belladonna . All of the nine transgenic hairy root lines ovexpressing both pmt and h6h produced tropane alkaloids at… Expand
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TLDR
It is concluded that transgenic plants Harboring both pmt and h6h possessed an increased flux in the tropane alkaloid biosynthetic pathway that enhanced scopolamine yield, which was more efficient than plants harboring only one of the two genes. Expand
Hairy root culture of Przewalskia tangutica for enhanced production of pharmaceutical tropane alkaloids
TLDR
Hairy root cultures of P. tangutica were established to produce tropane alkaloids for the first time, and produced scopolamine and hyoscyamine at higher levels by comparison to the wild-type roots. Expand
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Putrescine N-methyltransferase (PMT) is the first alkaloid-specific enzyme for nicotine and tropane alkaloid formation. The pmt gene from Nicotiana tabacum was fused to the CaMV 35S promoter andExpand
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TLDR
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TLDR
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TLDR
The hydroxylase gene from Hyoscyamus niger under the control of the cauliflower mosaic virus 35S promoter is introduced into hyoscyamine-rich Atropa belladonna by the use of an Agrobacterium-mediated transformation system and should prove useful as breeding materials for obtaining improved medicinal components. Expand
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TLDR
PMT was partially purified and characterized from cultured roots of Hyoscyamus albus that contain hyoscyamine as the main alkaloid and Monoamines were good competitive inhibitors of PMT, among which n-butylamine, cyclohexylamines, and exo-2-aminonorbornane were most inhibitory. Expand
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